Lomustine

카탈로그 번호S1840 배치:S184004

인쇄

기술 자료

화학식

C9H16ClN3O2
분자량 233.7 CAS 번호 13010-47-4
용해도 (25°C)* 시험관 내(In vitro) DMSO 47 mg/mL (201.11 mM)
Water Insoluble
Ethanol Insoluble
생체 내(In Vivo) (개별적으로 순서대로 용매를 제품에 첨가하십시오.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml은 약간 용해되거나 불용해됨을 의미합니다.
* Selleck은 모든 화합물의 용해도를 자체적으로 테스트하며, 실제 용해도는 게시된 값과 약간 다를 수 있습니다. 이는 정상적인 현상이며, 약간의 배치 간 변동으로 인해 발생합니다.
* 실온 배송 (안정성 테스트 결과 이 제품은 냉각 조치 없이 배송될 수 있음을 보여줍니다.)

원액 준비

생물학적 활성

설명 Lomustine (NSC79037, Gleostine, CeeNU, CCNU) inhibits cancer cells by damaging the DNA and stops cells from dividing.
시험관 내(In vitro) Lomustine inhibits the growth of ZR-75-1 and U373 with IC50 of 12 μM and 15 μM, respectively. This compound reduces the level of expression of the DNA repair protein O6-alkylguanine-DNA alkyltransferase. It (420 μM) triggers apoptosis through the mitochondrial pathway via decrease in the level of the anti-apoptosis proteins Bcl-2 and Bcl-xl, respectively, in both medulloblastoma and normal human epithelial and fibroblast cells. This chemical induces cell cycle delay in G2/M phase in medulloblastoma cells and up-regulates p21 protein level in a p53-independent manner in HFSN1 cells.
생체 내(In Vivo) Lomustine can cause delayed, cumulative dose-related, chronic hepatotoxicity that is irreversible and can be fatal. This compound could result in infrequent severe hematological toxicity in cats with spontaneously arising tumors, and the incidence of either grade III or IV neutropenia and thrombocytopenia is 4.1% and 1.0%, respectively. It trends toward a greater likelihood for progressive neutropenia and statistically significant higher response rates in cats with spontaneously arising tumors.
특징 A more specific and potent anti-medulloblastoma agent compared to Vincristine.

프로토콜 (참조)

세포 분석:[1]
  • 세포주

    XP cells

  • 농도

    133 μM

  • 배양 시간

    1 hour

  • 방법

    Cell lines are routinely grown as monolayers in DMEM supplemented with 10% foetal calf serum, 25 mm HEPES, glutamine and penicillin/streptomycin. Cytotoxicity studies are carried out in HEPES-free medium in a 5% CO2 atmosphere. 750-1000 cells/well are plated in 96 well plates and after overnight incubation are treated for 2 hours with or without 33 μM BG. Temozolomide or this compound is then added for 1 hour in the same medium, the final DMSO concentration not exceeding 1%. The cells are grown for a further 7 days in fresh medium and assayed for protein content by the NCI sulphorhodamine assay; growth studies show that cells are in log phase growth during the assay period. For the repeat temozolomide dosing schedule cells are given consecutive 24 hours treatments, with fresh medium each day. Assays are carried out at least in duplicate.
동물 연구:[3]
  • 동물 모델

    Dogs

  • 용량

    90 mg/m2

  • 투여

    Orally

참조

  • https://pubmed.ncbi.nlm.nih.gov/8512814/
  • https://pubmed.ncbi.nlm.nih.gov/18058069/
  • https://pubmed.ncbi.nlm.nih.gov/10587263/
  • https://pubmed.ncbi.nlm.nih.gov/14765735/
  • https://pubmed.ncbi.nlm.nih.gov/12118689/

고객 제품 검증

<p>Initial range finding concentration response analysis of the cytotoxic effects of (A) lomustine (IC50 = 12 μM) and (B) vincristine (IC50 = 1.5 nM) on D283 Med in a clonogenic assay of cell survival</p>

, , J Cell Mol Med, 2017, 21(12):3337-3346

Selleck's Lomustine 인용됨 23 출판물

CDKN2A/B Homozygous Deletion Sensitizes IDH-Mutant Glioma to CDK4/6 Inhibition [ Clinical Cancer Research, July 15, 2024, 2996-3005] PubMed: 38718141
Combinatorial treatment of glioblastoma with temozolomide (TMZ) plus 5-ethynyl-2-deoxyuridine (EdU) [ Proceedings of the National Academy of Sciences, January 6, 2026, e2532187123] PubMed: 41474743
Multiplexed epigenetic memory editing using CRISPRoff sensitizes glioblastoma to chemotherapy [ Neuro Oncol, 2025, noaf055] PubMed: 39998382
Isotretinoin promotes elimination of translation-competent HIV latent reservoirs in CD4T cells [ PLoS Pathog, 2024, 20(10):e1012601] PubMed: 39401241
Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862] PubMed: 39319271
Inhibition of epigenetic and cell cycle-related targets in glioblastoma cell lines reveals that onametostat reduces proliferation and viability in both normoxic and hypoxic conditions [ Sci Rep, 2024, 14(1):4303] PubMed: 38383756
A living ex vivo platform for functional, personalized brain cancer diagnosis [ Cell Rep Med, 2023, S2666-3791(23)00156-8] PubMed: 37192626
Mitotic DNA damage promotes chromokinesin-mediated missegregation of polar chromosomes in cancer cells [ Mol Biol Cell, 2023, 34(5):ar47] PubMed: 36989031
Establishment and Characterization of NCC-PMP1-C1: A Novel Patient-Derived Cell Line of Metastatic Pseudomyxoma Peritonei [ J Pers Med, 2022, 12(2)258] PubMed: 35207746
Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma [ Life Sci Alliance, 2022, 5(6)e202201380] PubMed: 35296518

반품 정책
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배송 및 보관
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