Home Stem Cells & Wnt activator ISX-9 (Isoxazole 9)

연구용

ISX-9 (Isoxazole 9) Neural Stem Cell Differentiation Inducer

제품 번호S7914

Isoxazole 9 (Isx-9) is a synthetic promotor of adult neurogenesis by triggering neuronal differentiation of adult neural stem/precursor cells (NSPCs). Isoxazole 9 (Isx-9) activates multiple pathways including TGF-β induced epithelial–mesenchymal transition (EMT) signaling, canonical and non-canonical Wnt signaling at different stages of cardiac differentiation.
ISX-9 (Isoxazole 9) activator Chemical Structure

화학 구조

분자량: 234.27

바로가기

품질 관리 (Quality Control)

배치: 순도: 99.87%
99.87

세포 배양, 처리 및 작업 농도
(Cell Culture, Treatment & Working Concentration)

세포주 분석 유형 농도 배양 시간 제형 활성 설명 PMID
HCN Function assay Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 5 uM Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of multiple of inhibitors 18552832
HCN Function assay 50 uM 3 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 25 uM 4 days Inhibition of gliogenesis differentiation in rat HCN cells at 25 uM after 4 days 18552832
HCN Function assay 5 uM Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis 18552832
HCN Function assay 5 uM Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 50 uM 3 hrs Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of multiple inhibitors 18552832
HCN Function assay Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells 18552832
HCN Function assay Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence multiple inhibitors 18552832
HCN Function assay 50 uM 24 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 50 uM 24 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay 50 uM 24 hrs Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of multiple inhibitors 18552832
HCN Function assay 8 to 64 uM 9 hrs Induction of neurogenesis in undifferentiated rat HCN cells assessed as MAP2AB protein level at 8 to 64 uM after 9 hrs by protein blotting analysis 18552832
HCN Function assay Induction of HDAC5 translocation in cytoplasm of rat HCN cells assessed as phosphorylated HDAC5 accumulation 18552832
HCN Function assay Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 2.5 uM potassium channel inhibitor KN92 18552832
HCN Function assay 8 to 64 uM 9 hrs Induction of neurogenesis in undifferentiated rat HCN cells assessed as GlR2/3 protein level at 8 to 64 uM after 9 hrs by protein blotting analysis 18552832
HCN Function assay 6 hrs Induction of HDAC5 phosphorylation in rat HCN cells after 6 hrs 18552832
HCN Function assay 24 hrs Induction of HDAC5 phosphorylation in rat HCN cells after 24 hrs 18552832
HCN Function assay Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as phosphorylated HDAC5 accumulation 18552832
HCN Function assay 5 uM Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 S258A S498A mutant fusion protein accumulation at 5 uM by fluorescence assay 18552832
HCN Function assay 50 uM 3 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay 20 uM Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 fusion protein accumulation at 20 uM by fluorescence assay 18552832
HCN Function assay Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 200 nM PKC inhibitor Go6976 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of multiple inhibitors 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist nifedipine 18552832
클릭하여 더 많은 세포주 실험 데이터 보기

화학 정보, 보관 및 안정성 (Chemical Information, Storage & Stability)

분자량 234.27 화학식

C11H10N2O2S

 보관 (수령일로부터)
CAS 번호 832115-62-5 SDF 다운로드 원액 보관

동의어 N/A Smiles C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3

용해도 (Solubility)

In vitro
배치:

DMSO : 47 mg/mL (200.62 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Ethanol : 12 mg/mL

Water : Insoluble

몰농도 계산기

질량 농도 부피 분자량
희석 계산기 분자량 계산기

In vivo
배치:

생체 내 제형 계산기 (투명한 용액)

1단계: 아래 정보 입력 (권장: 실험 중 손실을 고려하여 추가 동물 포함)

mg/kg g μL

2단계: 생체 내 제형 입력 (이것은 계산기일 뿐 제형이 아닙니다. 용해도 섹션에 생체 내 제형이 없는 경우 먼저 당사에 문의하십시오.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

계산 결과:

작업 농도: mg/ml;

DMSO 원액 준비 방법: mg 약물 사전 용해 μL DMSO ( 원액 농도 mg/mL, 농도가 해당 약물 배치의 DMSO 용해도를 초과하는 경우 먼저 당사에 문의하십시오. )

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가μL PEG300, 혼합하고 투명하게 한 다음 추가μL Tween 80, 혼합하고 투명하게 한 다음 추가 μL ddH2O, 혼합하고 투명하게 합니다.

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가 μL 옥수수 기름, 혼합하고 투명하게 합니다.

참고: 1. 다음 용매를 추가하기 전에 액체가 투명한지 확인하십시오.
2. 용매를 순서대로 추가해야 합니다. 다음 용매를 추가하기 전에 이전 추가에서 얻은 용액이 투명한 용액인지 확인해야 합니다. 와동, 초음파 또는 뜨거운 물 중탕과 같은 물리적 방법을 사용하여 용해를 도울 수 있습니다.

작용 메커니즘 (Mechanism of Action)

시험관 내(In vitro)
Isoxazole 9 (ISX-9) increases cell number and promotes cell differentiation in NSPCs, whereas it induces cell damage in OPCs. In outgrowth EPCs, this compound decreases tube formation without effect on early EPCs.
생체 내(In vivo)
In mice, isoxazole 9 (ISX-9) (20 mg/kg, i.p.) crosses the BBB and increases proliferation of neuroblasts and neurogenesis via Mef2-specific mechanisms in the hippocampal SGZ. This compound also increases differentiation and dendritic complexity of immature neurons and improves memory. In MWM, it improves spatial memory.
참조