Home Endocrinology & Hormones PGES 화학 Prostaglandin E2 (PGE2)

연구용

Prostaglandin E2 (PGE2) Prostaglandin E Receptor 작용제

제품 번호: S3003

Prostaglandin E2 (PGE2, Dinoprostone)은 분만(자궁경부 연화 및 자궁 수축 유발)에 중요한 역할을 하며, 골아세포를 자극하여 파골세포에 의한 골 흡수를 촉진하는 인자를 방출하게 합니다. 액체 상태로 장기간 보관하면 변색되기 쉽습니다. 분말 형태로 보관하고 가능한 한 빨리 사용하는 것을 권장합니다!
Prostaglandin E2 (PGE2) PGES 화학 Chemical Structure

화학 구조

분자량: 352.47

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품질 관리 (Quality Control)

배치: 순도: >97.00%
97.00

세포 배양, 처리 및 작업 농도
(Cell Culture, Treatment & Working Concentration)

세포주 분석 유형 농도 배양 시간 제형 활성 설명 PMID
HeLa Function assay TP_TRANSPORTER: uptake in PGT-expressing HeLa cells, Km = 0.094 μM. 7754369
HeLa Function assay TP_TRANSPORTER: uptake in PGT-expressing HeLa cells, K1/2 = 0.1 μM. 8787677
S2 Function assay TP_TRANSPORTER: uptake in OCT2-expressing S2 cells, Km = 0.0289 μM. 11907186
S2 Function assay TP_TRANSPORTER: uptake in OAT4-expressing S2 cells, Km = 0.154 μM. 11907186
S2 Function assay TP_TRANSPORTER: uptake in OAT3-expressing S2 cells, Km = 0.345 μM. 11907186
S2 Function assay TP_TRANSPORTER: uptake in OCT1-expressing S2 cells, Km = 0.657 μM. 11907186
S2 Function assay TP_TRANSPORTER: uptake in OAT2-expressing S2 cells, Km = 0.713 μM. 11907186
S2 Function assay TP_TRANSPORTER: uptake in OAT1-expressing S2 cells, Km = 0.97 μM. 11907186
HEK293 Function assay Inhibitory activity against human EP4 receptor expressed in HEK293 ebna cells, IC50 = 0.0007 μM. 12643927
HEK293 Function assay EP4 agonist potency utilizing a stable clone of pSV40-EP4 transfected into HEK293 cells expressing EP4 receptor, EC50 = 0.003 μM. 12643927
Sf9 Function assay TP_TRANSPORTER: uptake (vesicle) in membrane vesicles from MRP4-expressing Sf9 cells, Km = 3.4 μM. 12835412
HEK293 Function assay Displacement of [3H]PGE2 from human EP3 receptor expressed in HEK293 cells, Ki = 0.00033 μM. 17531488
HEK293 Function assay Displacement of [3H]PGE2 from human EP4 receptor expressed in HEK293 cells, Ki = 0.00079 μM. 17531488
HEK293 Function assay Displacement of [3H]PGE2 from human EP2 receptor expressed in HEK293 cells, Ki = 0.0049 μM. 17531488
HEK293 Function assay Displacement of [3H]PGE2 from human EP1 receptor expressed in HEK293 cells, Ki = 0.0091 μM. 17531488
HEK293 Function assay Agonist activity against rat EP2 receptor expressed in HEK293 cells assessed as stimulation of cAMP release, EC50 = 0.0002 μM. 19250823
HEK293 Function assay Agonist activity against rat EP4 receptor expressed in HEK293 cells assessed as stimulation of cAMP release, EC50 = 0.0007 μM. 19250823
HEK293 Function assay Inhibition of rat EP4 receptor expressed in HEK293 cells, IC50 = 0.0021 μM. 19250823
HEK293 Function assay Inhibition of rat EP2 receptor expressed in HEK293 cells, IC50 = 0.0052 μM. 19250823
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP4 receptor expressed in CHO cells after 60 mins by liquid scintillation counting, Ki = 0.0031 μM. 22119471
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP3 receptor expressed in CHO cells after 60 mins by liquid scintillation counting, Ki = 0.005 μM. 22119471
CHO Function assay 20 mins Displacement of [3H]-PGE2 from mouse EP1 receptor expressed in CHO cells after 20 mins by liquid scintillation counting, Ki = 0.006 μM. 22119471
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP2 receptor expressed in CHO cells after 60 mins by liquid scintillation counting, Ki = 0.022 μM. 22119471
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP4 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.0031 μM. 22386979
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP3 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.005 μM. 22386979
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP1 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.006 μM. 22386979
CHO Function assay 60 mins Displacement of [3H]-PGE2 from mouse EP2 receptor expressed in CHO cells after 60 mins by scintillation counting, Ki = 0.022 μM. 22386979
CHEM1 Function assay 2 hrs Displacement of [3H]PGE2 from human recombinant prostanoid EP4 receptor in CHEM1 cells after 2 hrs, Ki = 0.00045 μM. 23403082
CHEM1 Function assay 2 hrs Displacement of [3H]PGE2 from human recombinant prostanoid EP4 receptor in CHEM1 cells after 2 hrs, IC50 = 0.0011 μM. 23403082
CHO Function assay 100 nM Activity at recombinant EP1 receptor (unknown origin) expressed in CHO cells co-expressing Gq protein at 100 nM by electrical cell substrate impedance sensing system 25701254
CHO Function assay 100 nM Activity at recombinant EP4 receptor (unknown origin) expressed in CHO cells co-expressing Gs protein at 100 nM by electrical cell substrate impedance sensing system 25701254
CHO Function assay 100 nM Activity at recombinant EP3 receptor (unknown origin) expressed in CHO cells co-expressing Gi protein at 100 nM by electrical cell substrate impedance sensing system 25701254
CHO Function assay 30 mins Agonist activity at human EP2 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method, EC50 = 0.0019 μM. 26985320
CHO Function assay Agonist activity at human EP3 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis, EC50 = 0.0025 μM. 26985320
CHO Function assay Agonist activity at human EP1 receptor expressed in CHO cells assessed as increase in intracellular calcium level by fluorescence based analysis, EC50 = 0.0037 μM. 26985320
CHO Function assay 30 mins Agonist activity at human EP4 receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method, EC50 = 0.0075 μM. 26985320
Chem1 Function assay Agonist activity at human FP receptor expressed in human Chem1 cells assessed as increase in intracellular calcium level by fluorescence based analysis, EC50 = 0.25 μM. 26985320
HEK293 Function assay 90 mins Agonist activity at PK2-tagged human EP2 receptor expressed in HEK293 cells assessed as induction of EA-tagged beta-arrestin recruitment incubated for 90 mins by beta-galactosidase reporter gene assay, EC50 = 0.346 μM. 26985320
CHO Function assay 30 mins Agonist activity at human IP receptor expressed in CHO cells assessed as increase in intracellular cAMP level after 30 mins by HTRF method, EC50 = 0.347 μM. 26985320
HEK293 Function assay Displacement of [3H]PGE2 from human recombinant prostanoid EP2 receptor expressed in HEK293 cells, IC50 = 0.0026 μM. 26988801
HEK293 Function assay 120 mins Displacement of [3H]PGE2 from human recombinant EP4 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method, IC50 = 0.00055 μM. 27876250
HEK293 Function assay 120 mins Displacement of [3H]PGE2 from human recombinant EP2 receptor expressed in HEK293 cells measured after 120 mins by scintillation counting method, IC50 = 0.0026 μM. 27876250
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화학 정보, 보관 및 안정성 (Chemical Information, Storage & Stability)

분자량 352.47 화학식

C20H32O5

 보관 (수령일로부터)
CAS 번호 363-24-6 SDF 다운로드 원액 보관 용액은 불안정합니다. 신선하게 준비하거나 소량의 사전 포장된 크기를 구매하십시오. 수령 즉시 재포장하십시오.
동의어 Dinoprostone Smiles CCCCCC(C=CC1C(CC(=O)C1CC=CCCCC(=O)O)O)O

용해도 (Solubility)

In vitro
배치:

DMSO : 70 mg/mL (198.59 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Ethanol : 70 mg/mL

Water : 2.5 mg/mL

몰농도 계산기

질량 농도 부피 분자량
희석 계산기 분자량 계산기

In vivo
배치:

생체 내 제형 계산기 (투명한 용액)

1단계: 아래 정보 입력 (권장: 실험 중 손실을 고려하여 추가 동물 포함)

mg/kg g μL

2단계: 생체 내 제형 입력 (이것은 계산기일 뿐 제형이 아닙니다. 용해도 섹션에 생체 내 제형이 없는 경우 먼저 당사에 문의하십시오.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

계산 결과:

작업 농도: mg/ml;

DMSO 원액 준비 방법: mg 약물 사전 용해 μL DMSO ( 원액 농도 mg/mL, 농도가 해당 약물 배치의 DMSO 용해도를 초과하는 경우 먼저 당사에 문의하십시오. )

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가μL PEG300, 혼합하고 투명하게 한 다음 추가μL Tween 80, 혼합하고 투명하게 한 다음 추가 μL ddH2O, 혼합하고 투명하게 합니다.

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가 μL 옥수수 기름, 혼합하고 투명하게 합니다.

참고: 1. 다음 용매를 추가하기 전에 액체가 투명한지 확인하십시오.
2. 용매를 순서대로 추가해야 합니다. 다음 용매를 추가하기 전에 이전 추가에서 얻은 용액이 투명한 용액인지 확인해야 합니다. 와동, 초음파 또는 뜨거운 물 중탕과 같은 물리적 방법을 사용하여 용해를 도울 수 있습니다.

작용 메커니즘 (Mechanism of Action)

시험관 내(In vitro)

PGE2 acts via EP4 receptors on osteoclasts during the progression of OA and OA-related pain.
생체 내(In vivo)

PGE2 (0.3 μg/k, i.p.) significantly reduces the number of peritoneab macrophages undergoing phagocytosis of the methacrybate microbeads in rats.
참조

임상시험 정보 (Clinical Trial Information)

(데이터 출처 https://clinicaltrials.gov, 업데이트 날짜 2024-05-22)

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