ACBI1

ACBI1은 BAF ATPase 서브유닛 SMARCA2 및 SMARCA4의 강력한 PROTAC 분해제이며, MV-4-11 세포에서 SMARCA2, SMARCA4 및 PBRM1에 대해 각각 6 nM, 11 nM 및 32 nM의 DC50으로 폴리브로모 관련 BAF (PBAF) 복합체 구성원 PBRM1도 분해합니다. 이 화합물은 항증식 효과 및 세포자멸사를 유도할 수 있습니다.^{<a class="sref" href="#s_ref">[1]</a>}

• 세포주

  MV-4-11 cells

• 농도

  0.1-1000 nM

• 배양 시간

  18 h

• 방법

  ACBI1 is diluted from a 10 mM DMSO stock solution to 20 μM in buffer consisting of 20 mM 1,3-bis[tris(hydroxymethyl)methylamino] propane (BIS-TRIS) propane, 150 mM NaCl, 1 mM TCEP, pH 7.4 and a final DMSO concentration of 0.2%. Two sets of sequential titrations aer performed; first, a titration of SMARCA2BD (200 μM, in the syringe) into this compound solution (20 μM, in the cell), followed by a titration of VCB complex solution (168 μM in the syringe) into the PROTAC–SMARCA2BD complex solution retained in the cell. Binary experiments consisted of an analogous initial single injection of buffer (syringe) into this chemical solution (20 μM, in the cell), followed by a titration of VCB complex solution (168 μM in the syringe) into the diluted PROTAC solution retained in the cells.