Lamivudine (3TC, BCH-189)

카탈로그 번호S1706 배치:S170603

인쇄

기술 자료

화학식

C8H11N3O3S
분자량 229.26 CAS 번호 134678-17-4
용해도 (25°C)* 시험관 내(In vitro) DMSO 46 mg/mL (200.64 mM)
Water 46 mg/mL (200.64 mM)
Ethanol Insoluble
생체 내(In Vivo) (개별적으로 순서대로 용매를 제품에 첨가하십시오.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml은 약간 용해되거나 불용해됨을 의미합니다.
* Selleck은 모든 화합물의 용해도를 자체적으로 테스트하며, 실제 용해도는 게시된 값과 약간 다를 수 있습니다. 이는 정상적인 현상이며, 약간의 배치 간 변동으로 인해 발생합니다.
* 실온 배송 (안정성 테스트 결과 이 제품은 냉각 조치 없이 배송될 수 있음을 보여줍니다.)

원액 준비

생물학적 활성

설명 Lamivudine is a potent nucleoside analog reverse transcriptase inhibitor, used for treatment of chronic HBV and HIV/AIDS. It works by blocking the HIV reverse transcriptase and hepatitis B virus polymerase.
표적
Reverse transcriptase
시험관 내(In vitro)

Lamivudine’s anti- HBV activity, like its anti-HIV activity, has been shown to depend on the ability of LMV-TP to serve as both substrate and inhibitor of the DNA- and RNA-dependent polymerase activities of the HBV P gene product. This compound owes its activity to the remarkably broad substrate specificity of deoxycytidine kinase and the unusual substrate preference of the HBV polymerases for dNTPs with the unnatural L-conformation, whereas the anti-HBV activity of PCV appears to depend on several factors including optimal phosphorylation (sufficient for antiviral activity but not cytotoxicity) by key cellular enzymes, the long intracellular half-life of PCV-TP and the ability of PCV-TP to inhibit the HBV RT priming reaction as well as RT and DNA polymerase activity.

This compound and Penciclovir inhibits duck hepatitis B virus (DHBV) replication to a comparable extent when used alone, and in combination, the two nucleoside analogs acts synergistically over a wide range of clinically relevant concentrations. It combined with Penciclovir is more effective in reducing the normally recalcitrant viral covalently closed circular (CCC) DNA form of DHBV than either drug alone.

It inhibits p24 antigen production by HIV-I in PBMC, with ED50s ranging from 0.07 μM to 0.2 μM.

프로토콜 (참조)

세포 분석:

[4]
  • 세포주

    Neonatal rat ventricular cardiomyocytes

  • 농도

    5 μM

  • 배양 시간

    24 h

  • 방법

    Cells were treated with indicated concentrations of this compound for 24 h.

참조

  • https://pubmed.ncbi.nlm.nih.gov/10607220/
  • https://pubmed.ncbi.nlm.nih.gov/9214473/
  • https://pubmed.ncbi.nlm.nih.gov/8568296/
  • https://pubmed.ncbi.nlm.nih.gov/33898535/
  • https://www.sciencedirect.com/science/article/pii/S1818087616000131

고객 제품 검증

The indicated liver cancer cell lines—Hep-3B, Huh-7 and PLC were treated with or without 5 types anti-hepatitis B virus drugs (C) with the concentration of 100 μM, and M1 virus (MOI = 10) for 72 hours. Following 72 hours, cell viabilities were determined by MTT assay (mean ± SD). N.S. Not significant.

데이터 출처 [ , , Oncotarget, 2017, 8(15):24694-24705 ]

Selleck's Lamivudine (3TC, BCH-189) 인용됨 31 출판물

LINE-1 ORF1p Mimics Viral Innate Immune Evasion Mechanisms in Pancreatic Ductal Adenocarcinoma [ Cancer Discovery, May 02 2025, 1063-1082] PubMed: 39919290
Antiretroviral Drugs Regulate Epigenetic Modification of Cardiac Cells Through Modulation of H3K9 and H3K27 Acetylation [ Frontiers in Cardiovascular Medicine, April 09 2021, 634774] PubMed: 33898535
LINE-1 ORF1p Mimics Viral Innate Immune Evasion Mechanisms in Pancreatic Ductal Adenocarcinoma [ Cancer Discov, 2025, 10.1158/2159-8290.CD-24-1317] PubMed: 39919290
HELQ Maintains Genome Stability of Primordial Germ Cells by Inhibiting LINE-1 Expression [ FASEB J, 2025, 39(12):e70720] PubMed: 40542648
In vitro and patient studies with platelets to explore off-target cardiovascular effects of integrase inhibitors [ HIV Med, 2025, 26(2):285-294] PubMed: 39544128
Dietary nucleic acids promote oral tolerance through innate sensing pathways in mice [ Nat Commun, 2024, 15(1):9461] PubMed: 39487135
Inhibiting EZH2 targets atypical teratoid rhabdoid tumor by triggering viral mimicry via both RNA and DNA sensing pathways [ Nat Commun, 2024, 15(1):9321] PubMed: 39472584
Significance of hepatitis B virus capsid dephosphorylation via polymerase [ J Biomed Sci, 2024, 31(1):34] PubMed: 38561844
Preclinical and clinical antiviral characterization of AB-836, a potent capsid assembly modulator against hepatitis B virus [ Antiviral Res, 2024, 231:106010] PubMed: 39326502
Rupestonic Acid Derivative YZH-106 Promotes Lysosomal Degradation of HBV L- and M-HBsAg via Direct Interaction with PreS2 Domain [ Viruses, 2024, 16(7)1151] PubMed: 39066313

반품 정책
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