Home PI3K/Akt/mTOR mTOR inhibiteur Torin 1

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Torin 1 mTOR inhibiteur

N° Cat.S2827

Torin 1 est un puissant inhibiteur de mTORC1/2 avec un IC50 de 2 nM/10 nM dans les essais sans cellules; il présente une sélectivité 1000 fois supérieure pour mTOR que pour PI3K.
Torin 1 mTOR inhibiteur Chemical Structure

Structure chimique

Poids moléculaire: 607.62

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Contrôle qualité

Lot : Pureté : 99.07%
99.07

Culture cellulaire, traitement et concentration de travail

Lignées cellulaires Type dessai Concentration Temps dincubation Formulation Description de lactivité PMID
ETK-1 Growth Inhibition Assay IC50=0.000306 μM SANGER
OCUB-M Growth Inhibition Assay IC50=0.00194 μM SANGER
DOHH-2 Growth Inhibition Assay IC50=0.00225 μM SANGER
NOS-1 Growth Inhibition Assay IC50=0.00242 μM SANGER
L-363 Growth Inhibition Assay IC50=0.00284 μM SANGER
RL95-2 Growth Inhibition Assay IC50=0.0032 μM SANGER
BL-70 Growth Inhibition Assay IC50=0.00337 μM SANGER
TGBC1TKB Growth Inhibition Assay IC50=0.00379 μM SANGER
697 Growth Inhibition Assay IC50=0.00634 μM SANGER
RPMI-8226 Growth Inhibition Assay IC50=0.00651 μM SANGER
BL-41 Growth Inhibition Assay IC50=0.00696 μM SANGER
MRK-nu-1 Growth Inhibition Assay IC50=0.00707 μM SANGER
EW-1 Growth Inhibition Assay IC50=0.00749 μM SANGER
EVSA-T Growth Inhibition Assay IC50=0.00892 μM SANGER
HUTU-80 Growth Inhibition Assay IC50=0.00929 μM SANGER
VA-ES-BJ Growth Inhibition Assay IC50=0.00984 μM SANGER
GCIY Growth Inhibition Assay IC50=0.01007 μM SANGER
ES6 Growth Inhibition Assay IC50=0.01083 μM SANGER
NALM-6 Growth Inhibition Assay IC50=0.01102 μM SANGER
LB996-RCC Growth Inhibition Assay IC50=0.01137 μM SANGER
EW-16 Growth Inhibition Assay IC50=0.01192 μM SANGER
KALS-1 Growth Inhibition Assay IC50=0.01198 μM SANGER
OPM-2 Growth Inhibition Assay IC50=0.0129 μM SANGER
KU812 Growth Inhibition Assay IC50=0.01296 μM SANGER
A4-Fuk Growth Inhibition Assay IC50=0.01319 μM SANGER
KGN Growth Inhibition Assay IC50=0.01359 μM SANGER
KS-1 Growth Inhibition Assay IC50=0.01392 μM SANGER
no-11 Growth Inhibition Assay IC50=0.01395 μM SANGER
PSN1 Growth Inhibition Assay IC50=0.01409 μM SANGER
SK-LMS-1 Growth Inhibition Assay IC50=0.01413 μM SANGER
LS-411N Growth Inhibition Assay IC50=0.01439 μM SANGER
HT-144 Growth Inhibition Assay IC50=0.01451 μM SANGER
ACN Growth Inhibition Assay IC50=0.015 μM SANGER
RKO Growth Inhibition Assay IC50=0.01533 μM SANGER
CMK Growth Inhibition Assay IC50=0.01543 μM SANGER
GB-1 Growth Inhibition Assay IC50=0.01553 μM SANGER
KE-37 Growth Inhibition Assay IC50=0.01573 μM SANGER
no-10 Growth Inhibition Assay IC50=0.01584 μM SANGER
MOLT-16 Growth Inhibition Assay IC50=0.01622 μM SANGER
HEL Growth Inhibition Assay IC50=0.0164 μM SANGER
NCI-H1355 Growth Inhibition Assay IC50=0.01646 μM SANGER
IST-MEL1 Growth Inhibition Assay IC50=0.01774 μM SANGER
MHH-PREB-1 Growth Inhibition Assay IC50=0.01818 μM SANGER
ES8 Growth Inhibition Assay IC50=0.01844 μM SANGER
LC-2-ad Growth Inhibition Assay IC50=0.02137 μM SANGER
LAMA-84 Growth Inhibition Assay IC50=0.02167 μM SANGER
BV-173 Growth Inhibition Assay IC50=0.02203 μM SANGER
SW872 Growth Inhibition Assay IC50=0.02247 μM SANGER
SK-UT-1 Growth Inhibition Assay IC50=0.02261 μM SANGER
OS-RC-2 Growth Inhibition Assay IC50=0.02323 μM SANGER
NCI-H747 Growth Inhibition Assay IC50=0.0233 μM SANGER
MZ2-MEL Growth Inhibition Assay IC50=0.02337 μM SANGER
TE-11 Growth Inhibition Assay IC50=0.02372 μM SANGER
KURAMOCHI Growth Inhibition Assay IC50=0.02414 μM SANGER
HD-MY-Z Growth Inhibition Assay IC50=0.02426 μM SANGER
GI-1 Growth Inhibition Assay IC50=0.02469 μM SANGER
LB1047-RCC Growth Inhibition Assay IC50=0.02488 μM SANGER
TE-12 Growth Inhibition Assay IC50=0.0255 μM SANGER
MZ7-mel Growth Inhibition Assay IC50=0.02609 μM SANGER
MMAC-SF Growth Inhibition Assay IC50=0.02633 μM SANGER
A101D Growth Inhibition Assay IC50=0.0265 μM SANGER
GR-ST Growth Inhibition Assay IC50=0.02655 μM SANGER
MC116 Growth Inhibition Assay IC50=0.0268 μM SANGER
Ramos-2G6-4C10 Growth Inhibition Assay IC50=0.0275 μM SANGER
ATN-1 Growth Inhibition Assay IC50=0.02789 μM SANGER
MFH-ino Growth Inhibition Assay IC50=0.02812 μM SANGER
DEL Growth Inhibition Assay IC50=0.02815 μM SANGER
OCI-AML2 Growth Inhibition Assay IC50=0.02871 μM SANGER
LXF-289 Growth Inhibition Assay IC50=0.02872 μM SANGER
IA-LM Growth Inhibition Assay IC50=0.02917 μM SANGER
EoL-1-cell Growth Inhibition Assay IC50=0.02996 μM SANGER
OVCAR-4 Growth Inhibition Assay IC50=0.03094 μM SANGER
ES1 Growth Inhibition Assay IC50=0.03097 μM SANGER
CESS Growth Inhibition Assay IC50=0.03099 μM SANGER
GAK Growth Inhibition Assay IC50=0.03186 μM SANGER
NTERA-S-cl-D1 Growth Inhibition Assay IC50=0.03226 μM SANGER
TE-10 Growth Inhibition Assay IC50=0.03311 μM SANGER
CCRF-CEM Growth Inhibition Assay IC50=0.03371 μM SANGER
NMC-G1 Growth Inhibition Assay IC50=0.03448 μM SANGER
HOP-62 Growth Inhibition Assay IC50=0.03556 μM SANGER
SH-4 Growth Inhibition Assay IC50=0.03582 μM SANGER
NCI-H1648 Growth Inhibition Assay IC50=0.03622 μM SANGER
EB-3 Growth Inhibition Assay IC50=0.03643 μM SANGER
BB65-RCC Growth Inhibition Assay IC50=0.03691 μM SANGER
BE-13 Growth Inhibition Assay IC50=0.03759 μM SANGER
CP67-MEL Growth Inhibition Assay IC50=0.03759 μM SANGER
SW954 Growth Inhibition Assay IC50=0.0379 μM SANGER
TE-5 Growth Inhibition Assay IC50=0.03854 μM SANGER
HCE-4 Growth Inhibition Assay IC50=0.03855 μM SANGER
LC-1F Growth Inhibition Assay IC50=0.03989 μM SANGER
TK10 Growth Inhibition Assay IC50=0.04019 μM SANGER
SJSA-1 Growth Inhibition Assay IC50=0.04188 μM SANGER
Daudi Growth Inhibition Assay IC50=0.04225 μM SANGER
SW982 Growth Inhibition Assay IC50=0.04246 μM SANGER
SF126 Growth Inhibition Assay IC50=0.04361 μM SANGER
TE-15 Growth Inhibition Assay IC50=0.04435 μM SANGER
COLO-684 Growth Inhibition Assay IC50=0.04561 μM SANGER
8-MG-BA Growth Inhibition Assay IC50=0.04629 μM SANGER
NB13 Growth Inhibition Assay IC50=0.04776 μM SANGER
HCC1599 Growth Inhibition Assay IC50=0.04879 μM SANGER
SK-PN-DW Growth Inhibition Assay IC50=0.04949 μM SANGER
ALL-PO Growth Inhibition Assay IC50=0.04956 μM SANGER
NB6 Growth Inhibition Assay IC50=0.05271 μM SANGER
IST-SL2 Growth Inhibition Assay IC50=0.05362 μM SANGER
BB30-HNC Growth Inhibition Assay IC50=0.0573 μM SANGER
SW962 Growth Inhibition Assay IC50=0.05763 μM SANGER
TGBC24TKB Growth Inhibition Assay IC50=0.05799 μM SANGER
KINGS-1 Growth Inhibition Assay IC50=0.05803 μM SANGER
HC-1 Growth Inhibition Assay IC50=0.05827 μM SANGER
RS4-11 Growth Inhibition Assay IC50=0.05938 μM SANGER
LS-123 Growth Inhibition Assay IC50=0.05942 μM SANGER
A3-KAW Growth Inhibition Assay IC50=0.06225 μM SANGER
SNB75 Growth Inhibition Assay IC50=0.06341 μM SANGER
SR Growth Inhibition Assay IC50=0.06374 μM SANGER
LOUCY Growth Inhibition Assay IC50=0.06394 μM SANGER
K5 Growth Inhibition Assay IC50=0.06403 μM SANGER
Becker Growth Inhibition Assay IC50=0.0642 μM SANGER
CTV-1 Growth Inhibition Assay IC50=0.06548 μM SANGER
KLE Growth Inhibition Assay IC50=0.06584 μM SANGER
MZ1-PC Growth Inhibition Assay IC50=0.06598 μM SANGER
MOLT-4 Growth Inhibition Assay IC50=0.06605 μM SANGER
MLMA Growth Inhibition Assay IC50=0.0678 μM SANGER
A388 Growth Inhibition Assay IC50=0.06893 μM SANGER
MV-4-11 Growth Inhibition Assay IC50=0.06995 μM SANGER
DSH1 Growth Inhibition Assay IC50=0.07173 μM SANGER
MONO-MAC-6 Growth Inhibition Assay IC50=0.07212 μM SANGER
ARH-77 Growth Inhibition Assay IC50=0.07278 μM SANGER
LB771-HNC Growth Inhibition Assay IC50=0.07549 μM SANGER
PF-382 Growth Inhibition Assay IC50=0.07569 μM SANGER
KM12 Growth Inhibition Assay IC50=0.0764 μM SANGER
HAL-01 Growth Inhibition Assay IC50=0.07658 μM SANGER
RPMI-8402 Growth Inhibition Assay IC50=0.07789 μM SANGER
OMC-1 Growth Inhibition Assay IC50=0.07804 μM SANGER
HT Growth Inhibition Assay IC50=0.08 μM SANGER
QIMR-WIL Growth Inhibition Assay IC50=0.08055 μM SANGER
KNS-42 Growth Inhibition Assay IC50=0.08203 μM SANGER
BC-3 Growth Inhibition Assay IC50=0.08204 μM SANGER
ES4 Growth Inhibition Assay IC50=0.08296 μM SANGER
NCI-H23 Growth Inhibition Assay IC50=0.0844 μM SANGER
LB2518-MEL Growth Inhibition Assay IC50=0.08696 μM SANGER
LC4-1 Growth Inhibition Assay IC50=0.08738 μM SANGER
SIG-M5 Growth Inhibition Assay IC50=0.08904 μM SANGER
LP-1 Growth Inhibition Assay IC50=0.08938 μM SANGER
TE-8 Growth Inhibition Assay IC50=0.09016 μM SANGER
K-562 Growth Inhibition Assay IC50=0.0912 μM SANGER
J-RT3-T3-5 Growth Inhibition Assay IC50=0.09153 μM SANGER
DU-4475 Growth Inhibition Assay IC50=0.09165 μM SANGER
RXF393 Growth Inhibition Assay IC50=0.09462 μM SANGER
NB5 Growth Inhibition Assay IC50=0.09625 μM SANGER
A704 Growth Inhibition Assay IC50=0.09741 μM SANGER
SF268 Growth Inhibition Assay IC50=0.09825 μM SANGER
UACC-257 Growth Inhibition Assay IC50=0.09833 μM SANGER
L-428 Growth Inhibition Assay IC50=0.1032 μM SANGER
ST486 Growth Inhibition Assay IC50=0.10606 μM SANGER
SK-MM-2 Growth Inhibition Assay IC50=0.10709 μM SANGER
COR-L279 Growth Inhibition Assay IC50=0.10832 μM SANGER
JiyoyeP-2003 Growth Inhibition Assay IC50=0.10843 μM SANGER
GT3TKB Growth Inhibition Assay IC50=0.10918 μM SANGER
HCE-T Growth Inhibition Assay IC50=0.1094 μM SANGER
SBC-1 Growth Inhibition Assay IC50=0.11119 μM SANGER
KNS-81-FD Growth Inhibition Assay IC50=0.11273 μM SANGER
NCI-H2126 Growth Inhibition Assay IC50=0.11394 μM SANGER
EM-2 Growth Inhibition Assay IC50=0.11529 μM SANGER
JAR Growth Inhibition Assay IC50=0.11598 μM SANGER
NB17 Growth Inhibition Assay IC50=0.11652 μM SANGER
LB647-SCLC Growth Inhibition Assay IC50=0.11705 μM SANGER
CAL-148 Growth Inhibition Assay IC50=0.118 μM SANGER
K052 Growth Inhibition Assay IC50=0.11851 μM SANGER
KARPAS-299 Growth Inhibition Assay IC50=0.12233 μM SANGER
KARPAS-422 Growth Inhibition Assay IC50=0.12432 μM SANGER
LOXIMVI Growth Inhibition Assay IC50=0.12515 μM SANGER
SCC-3 Growth Inhibition Assay IC50=0.12851 μM SANGER
LU-139 Growth Inhibition Assay IC50=0.12975 μM SANGER
COLO-320-HSR Growth Inhibition Assay IC50=0.13086 μM SANGER
ONS-76 Growth Inhibition Assay IC50=0.1313 μM SANGER
U-266 Growth Inhibition Assay IC50=0.13245 μM SANGER
HH Growth Inhibition Assay IC50=0.13535 μM SANGER
BB49-HNC Growth Inhibition Assay IC50=0.13587 μM SANGER
NCI-SNU-5 Growth Inhibition Assay IC50=0.13596 μM SANGER
C2BBe1 Growth Inhibition Assay IC50=0.13746 μM SANGER
ML-2 Growth Inhibition Assay IC50=0.13953 μM SANGER
SCH Growth Inhibition Assay IC50=0.13974 μM SANGER
NCI-H82 Growth Inhibition Assay IC50=0.14104 μM SANGER
MDA-MB-134-VI Growth Inhibition Assay IC50=0.14252 μM SANGER
SK-N-DZ Growth Inhibition Assay IC50=0.14719 μM SANGER
HL-60 Growth Inhibition Assay IC50=0.1492 μM SANGER
LB373-MEL-D Growth Inhibition Assay IC50=0.15539 μM SANGER
LB831-BLC Growth Inhibition Assay IC50=0.16076 μM SANGER
SK-NEP-1 Growth Inhibition Assay IC50=0.16101 μM SANGER
IMR-5 Growth Inhibition Assay IC50=0.16483 μM SANGER
Calu-6 Growth Inhibition Assay IC50=0.16537 μM SANGER
NCI-H1882 Growth Inhibition Assay IC50=0.16728 μM SANGER
NCI-H446 Growth Inhibition Assay IC50=0.16891 μM SANGER
GI-ME-N Growth Inhibition Assay IC50=0.1802 μM SANGER
MPP-89 Growth Inhibition Assay IC50=0.18121 μM SANGER
CGTH-W-1 Growth Inhibition Assay IC50=0.18436 μM SANGER
MEG-01 Growth Inhibition Assay IC50=0.18628 μM SANGER
NCI-H748 Growth Inhibition Assay IC50=0.18911 μM SANGER
COLO-829 Growth Inhibition Assay IC50=0.20673 μM SANGER
GOTO Growth Inhibition Assay IC50=0.2073 μM SANGER
EW-3 Growth Inhibition Assay IC50=0.21718 μM SANGER
KY821 Growth Inhibition Assay IC50=0.22045 μM SANGER
CTB-1 Growth Inhibition Assay IC50=0.22228 μM SANGER
TALL-1 Growth Inhibition Assay IC50=0.22921 μM SANGER
NCI-H209 Growth Inhibition Assay IC50=0.22962 μM SANGER
IST-MES1 Growth Inhibition Assay IC50=0.2351 μM SANGER
DMS-114 Growth Inhibition Assay IC50=0.23993 μM SANGER
SNU-C2B Growth Inhibition Assay IC50=0.24316 μM SANGER
DG-75 Growth Inhibition Assay IC50=0.26195 μM SANGER
D-336MG Growth Inhibition Assay IC50=0.26557 μM SANGER
KMOE-2 Growth Inhibition Assay IC50=0.27538 μM SANGER
NB1 Growth Inhibition Assay IC50=0.27926 μM SANGER
RH-1 Growth Inhibition Assay IC50=0.27989 μM SANGER
TE-1 Growth Inhibition Assay IC50=0.28479 μM SANGER
RPMI-6666 Growth Inhibition Assay IC50=0.2884 μM SANGER
TE-9 Growth Inhibition Assay IC50=0.28985 μM SANGER
BT-474 Growth Inhibition Assay IC50=0.29334 μM SANGER
COLO-668 Growth Inhibition Assay IC50=0.29429 μM SANGER
NCI-H1581 Growth Inhibition Assay IC50=0.29689 μM SANGER
MN-60 Growth Inhibition Assay IC50=0.29905 μM SANGER
L-540 Growth Inhibition Assay IC50=0.30081 μM SANGER
MOLT-13 Growth Inhibition Assay IC50=0.30183 μM SANGER
NCI-SNU-16 Growth Inhibition Assay IC50=0.30205 μM SANGER
NCI-H64 Growth Inhibition Assay IC50=0.30331 μM SANGER
CP66-MEL Growth Inhibition Assay IC50=0.31415 μM SANGER
NB69 Growth Inhibition Assay IC50=0.32188 μM SANGER
NCI-H2196 Growth Inhibition Assay IC50=0.33036 μM SANGER
MHH-CALL-2 Growth Inhibition Assay IC50=0.33468 μM SANGER
HCC2157 Growth Inhibition Assay IC50=0.33954 μM SANGER
LS-1034 Growth Inhibition Assay IC50=0.36388 μM SANGER
BOKU Growth Inhibition Assay IC50=0.37199 μM SANGER
GDM-1 Growth Inhibition Assay IC50=0.37234 μM SANGER
TGW Growth Inhibition Assay IC50=0.37453 μM SANGER
SNU-C1 Growth Inhibition Assay IC50=0.38215 μM SANGER
LS-513 Growth Inhibition Assay IC50=0.39071 μM SANGER
NOMO-1 Growth Inhibition Assay IC50=0.39971 μM SANGER
MS-1 Growth Inhibition Assay IC50=0.40177 μM SANGER
EKVX Growth Inhibition Assay IC50=0.44461 μM SANGER
SK-MEL-2 Growth Inhibition Assay IC50=0.464 μM SANGER
HCC1187 Growth Inhibition Assay IC50=0.46768 μM SANGER
HCC2218 Growth Inhibition Assay IC50=0.47227 μM SANGER
NCI-H187 Growth Inhibition Assay IC50=0.4784 μM SANGER
DB Growth Inhibition Assay IC50=0.50055 μM SANGER
NCI-H69 Growth Inhibition Assay IC50=0.50247 μM SANGER
NCI-H1694 Growth Inhibition Assay IC50=0.50697 μM SANGER
CA46 Growth Inhibition Assay IC50=0.50917 μM SANGER
EHEB Growth Inhibition Assay IC50=0.51193 μM SANGER
NCCIT Growth Inhibition Assay IC50=0.54321 μM SANGER
NCI-H720 Growth Inhibition Assay IC50=0.54962 μM SANGER
LU-134-A Growth Inhibition Assay IC50=0.55296 μM SANGER
MC-CAR Growth Inhibition Assay IC50=0.55431 μM SANGER
NEC8 Growth Inhibition Assay IC50=0.56172 μM SANGER
NCI-H524 Growth Inhibition Assay IC50=0.58748 μM SANGER
NCI-H1963 Growth Inhibition Assay IC50=0.6258 μM SANGER
SU-DHL-1 Growth Inhibition Assay IC50=0.64526 μM SANGER
NCI-H1522 Growth Inhibition Assay IC50=0.64883 μM SANGER
NCI-H1155 Growth Inhibition Assay IC50=0.6535 μM SANGER
D-263MG Growth Inhibition Assay IC50=0.66101 μM SANGER
TE-6 Growth Inhibition Assay IC50=0.68104 μM SANGER
NCI-H1417 Growth Inhibition Assay IC50=0.69711 μM SANGER
NCI-H510A Growth Inhibition Assay IC50=0.77236 μM SANGER
KARPAS-45 Growth Inhibition Assay IC50=0.78235 μM SANGER
IST-SL1 Growth Inhibition Assay IC50=0.85825 μM SANGER
COLO-824 Growth Inhibition Assay IC50=0.87835 μM SANGER
NCI-H526 Growth Inhibition Assay IC50=0.90958 μM SANGER
NCI-H719 Growth Inhibition Assay IC50=0.96657 μM SANGER
SF539 Growth Inhibition Assay IC50=0.98396 μM SANGER
NKM-1 Growth Inhibition Assay IC50=0.98663 μM SANGER
A253 Growth Inhibition Assay IC50=0.98885 μM SANGER
TE-441-T Growth Inhibition Assay IC50=1.0435 μM SANGER
COR-L88 Growth Inhibition Assay IC50=1.11354 μM SANGER
CPC-N Growth Inhibition Assay IC50=1.15888 μM SANGER
NCI-H2107 Growth Inhibition Assay IC50=1.17261 μM SANGER
ECC4 Growth Inhibition Assay IC50=1.18693 μM SANGER
NCI-H2141 Growth Inhibition Assay IC50=1.19299 μM SANGER
NCI-H1770 Growth Inhibition Assay IC50=1.32928 μM SANGER
NCI-SNU-1 Growth Inhibition Assay IC50=1.3304 μM SANGER
LU-165 Growth Inhibition Assay IC50=1.45467 μM SANGER
Raji Growth Inhibition Assay IC50=1.47259 μM SANGER
EW-24 Growth Inhibition Assay IC50=1.54619 μM SANGER
KP-N-RT-BM-1 Growth Inhibition Assay IC50=1.56551 μM SANGER
SK-MEL-1 Growth Inhibition Assay IC50=1.64877 μM SANGER
SCLC-21H Growth Inhibition Assay IC50=1.66651 μM SANGER
SIMA Growth Inhibition Assay IC50=1.68233 μM SANGER
NCI-H1304 Growth Inhibition Assay IC50=1.70765 μM SANGER
REH Growth Inhibition Assay IC50=1.71078 μM SANGER
KP-N-YS Growth Inhibition Assay IC50=1.93551 μM SANGER
DMS-153 Growth Inhibition Assay IC50=1.9759 μM SANGER
NB10 Growth Inhibition Assay IC50=2.20878 μM SANGER
NCI-H345 Growth Inhibition Assay IC50=2.61905 μM SANGER
SK-N-FI Growth Inhibition Assay IC50=2.75381 μM SANGER
NCI-H1092 Growth Inhibition Assay IC50=2.95394 μM SANGER
KM-H2 Growth Inhibition Assay IC50=3.65396 μM SANGER
KG-1 Growth Inhibition Assay IC50=3.71688 μM SANGER
D-283MED Growth Inhibition Assay IC50=3.7587 μM SANGER
LU-65 Growth Inhibition Assay IC50=4.11485 μM SANGER
MSTO-211H Growth Inhibition Assay IC50=4.18898 μM SANGER
NCI-H2081 Growth Inhibition Assay IC50=4.29174 μM SANGER
D-247MG Growth Inhibition Assay IC50=4.43191 μM SANGER
P31-FUJ Growth Inhibition Assay IC50=4.45019 μM SANGER
DMS-79 Growth Inhibition Assay IC50=4.93183 μM SANGER
KASUMI-1 Growth Inhibition Assay IC50=4.97509 μM SANGER
JVM-3 Growth Inhibition Assay IC50=5.25014 μM SANGER
LAN-6 Growth Inhibition Assay IC50=5.51736 μM SANGER
DJM-1 Growth Inhibition Assay IC50=5.56029 μM SANGER
JVM-2 Growth Inhibition Assay IC50=5.67092 μM SANGER
U-87-MG Growth Inhibition Assay IC50=5.89964 μM SANGER
SHP-77 Growth Inhibition Assay IC50=5.94928 μM SANGER
RPMI-8866 Growth Inhibition Assay IC50=6.27013 μM SANGER
ES7 Growth Inhibition Assay IC50=6.39924 μM SANGER
WSU-NHL Growth Inhibition Assay IC50=6.51609 μM SANGER
CAS-1 Growth Inhibition Assay IC50=7.09749 μM SANGER
MHH-NB-11 Growth Inhibition Assay IC50=8.23152 μM SANGER
ES5 Growth Inhibition Assay IC50=9.52286 μM SANGER
HDLM-2 Growth Inhibition Assay IC50=9.95902 μM SANGER
NCI-H2171 Growth Inhibition Assay IC50=10.5927 μM SANGER
LNCaP-Clone-FGC Growth Inhibition Assay IC50=11.3718 μM SANGER
NCI-H1395 Growth Inhibition Assay IC50=12.2699 μM SANGER
NCI-H1436 Growth Inhibition Assay IC50=13.3195 μM SANGER
RCC10RGB Growth Inhibition Assay IC50=13.3221 μM SANGER
SW684 Growth Inhibition Assay IC50=13.9797 μM SANGER
NCI-H1650 Growth Inhibition Assay IC50=15.0447 μM SANGER
EW-13 Growth Inhibition Assay IC50=15.2021 μM SANGER
EC-GI-10 Growth Inhibition Assay IC50=15.8644 μM SANGER
CHP-126 Growth Inhibition Assay IC50=17.1918 μM SANGER
NCI-H226 Growth Inhibition Assay IC50=18.0403 μM SANGER
NCI-H1838 Growth Inhibition Assay IC50=20.1848 μM SANGER
EW-11 Growth Inhibition Assay IC50=24.2718 μM SANGER
EB2 Growth Inhibition Assay IC50=31.5318 μM SANGER
D-502MG Growth Inhibition Assay IC50=43.9229 μM SANGER
U-698-M Growth Inhibition Assay IC50=48.8671 μM SANGER
ES3 Growth Inhibition Assay IC50=49.3893 μM SANGER
PC3 Function assay Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM. 21322566
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells 29435139
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Informations chimiques, stockage et stabilité

Poids moléculaire 607.62 Formule

C35H28F3N5O2

 Stockage (À partir de la date de réception)
N° CAS 1222998-36-8 Télécharger le SDF Stockage des solutions mères

Synonymes N/A Smiles CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

Solubilité

In vitro
Lot:

DMSO : 3 mg/mL (4.93 mM)
(Le DMSO contaminé par lhumidité peut réduire la solubilité. Utiliser du DMSO frais et anhydre.)

Water : Insoluble

Ethanol : Insoluble

Calculateur de molarité

Masse Concentration Volume Poids moléculaire
Calculateur de dilution Calculateur de poids moléculaire

In vivo
Lot:

Calculateur de formulation in vivo (Solution claire)

Étape 1 : Entrez les informations ci-dessous (Recommandé : Un animal supplémentaire pour tenir compte des pertes pendant lexpérience)

mg/kg g μL

Étape 2 : Entrez la formulation in vivo (Ceci nest que le calculateur, pas la formulation. Veuillez nous contacter dabord sil ny a pas de formulation in vivo dans la section Solubilité.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Résultats du calcul :

Concentration de travail : mg/ml;

Méthode de préparation du liquide maître DMSO : mg médicament prédissous dans μL DMSO ( Concentration du liquide maître mg/mL, Veuillez nous contacter dabord si la concentration dépasse la solubilité du DMSO du lot de médicament. )

Méthode de préparation de la formulation in vivo : Prendre μL DMSO liquide maître, ajouter ensuiteμL PEG300, mélanger et clarifier, ajouter ensuiteμL Tween 80, mélanger et clarifier, ajouter ensuite μL ddH2O, mélanger et clarifier.

Méthode de préparation de la formulation in vivo : Prendre μL DMSO liquide maître, ajouter ensuite μL Huile de maïs, mélanger et clarifier.

Remarque : 1. Assurez-vous que le liquide est clair avant dajouter le solvant suivant.
2. Assurez-vous dajouter le(s) solvant(s) dans lordre. Vous devez vous assurer que la solution obtenue lors de lajout précédent est une solution claire avant de procéder à lajout du solvant suivant. Des méthodes physiques telles que le vortex, les ultrasons ou le bain-marie peuvent être utilisées pour faciliter la dissolution.

Mécanisme daction

Targets/IC50/Ki
mTORC1
(Cell-free assay)
2 nM
mTOR
(Cell-free assay)
4.32 nM
DNA-PK
(Cell-free assay)
6.34 nM
mTORC2
(Cell-free assay)
10 nM
p110γ
(Cell-free assay)
171 nM
C2α
(Cell-free assay)
176 nM
In vitro
Torin1 inhibe la phosphorylation des substrats de mTORC1 et mTORC2 dans les cellules à des concentrations de 2 et 10 nM, respectivement. De plus, Torin1 présente une sélectivité 1000 fois supérieure pour mTOR par rapport à PI3K (EC50 = 1800 nM) et une sélectivité de liaison 100 fois supérieure par rapport à 450 autres protéines kinases. Torin1 provoque un arrêt du cycle cellulaire via un mécanisme résistant à la rapamycine qui est également indépendant de mTORC2. Torin1 perturbe les phénotypes dépendants de mTORC1 plus complètement que la rapamycine. Les fonctions de mTORC1 résistantes à la rapamycine sont nécessaires pour la traduction dépendante de la coiffe. Dans une étude récente, il est rapporté que Torin1 augmente la sécrétion et l'expression génique de la neurotensine par l'activation de la voie MEK/ERK/c-Jun dans la lignée cellulaire endocrine humaine BON.
Kinase Assay
Tests de kinase in vitro de mTORC1 et mTORC2
Pour produire du mTORC1 soluble, des lignées cellulaires HEK-293T exprimant de manière stable le Raptor marqué FLAG en N-terminal sont générées à l'aide d'un rétrovirus MSCV pseudotypé par le virus de la stomatite vésiculeuse G. Pour mTORC2, des cellules HeLa similaires exprimant de manière stable le Protor-1 marqué FLAG en N-terminal sont générées. Les deux complexes sont purifiés en lysant les cellules dans 50 mm HEPES, pH 7,4, 10 mm pyrophosphate de sodium, 10 mm micro-glycérophosphate de sodium, 100 mm NaCl, 2 mm EDTA, 0,3% CHAPS. Les cellules sont lysées à 4 °C pendant 30 min, et la fraction insoluble est retirée par microcentrifugation à 13 000 tr/min pendant 10 min. Les surnageants sont incubés avec de l'anticorps monoclonal FLAG-M2-agarose pendant 1 h, puis lavés trois fois avec du tampon de lyse et une fois avec du tampon de lyse contenant une concentration finale de 0,5 M NaCl. Le mTORC1 purifié est élué avec 100 microg/mL de peptide 3 cFLAG dans 50 mm HEPES, pH 7,4, 100 mm NaCl. L'éluat peut être aliquote et stocké à -80 °C. Les substrats S6K1 et Akt1 sont purifiés. Les tests de kinase sont réalisés pendant 20 min à 30 °C dans un volume final de 20 microL composé du tampon de kinase (25 mm HEPES, pH 7,4, 50 mm KCl, 10 mm MgCl 2, 500 microM ATP) et de 150 ng de S6K1 ou Akt1 inactif comme substrats. Les réactions sont arrêtées par l'ajout de 80 microL de tampon d'échantillon et bouillies pendant 5 min. Les échantillons sont ensuite analysés par SDS-PAGE et immunobuvardage.
In vivo
Torin1 est efficace à une dose de 20 mg/kg dans un modèle de xénogreffe U87MG et démontre une bonne inhibition pharmacodynamique des effecteurs en aval de mTOR dans les tissus tumoraux et périphériques.
Références
  • [4] https://pubmed.ncbi.nlm.nih.gov/20508131/

Applications

Méthodes Biomarqueurs Images PMID
Western blot Vinculin / p-S6 p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b
S2827-WB4
28757207
Immunofluorescence lipin-1
S2827-IF1
28347999
Growth inhibition assay Cell viability
S2827-viability1
28757207

Support technique

Instructions de manipulation

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Questions fréquemment posées

Question 1:
How to re-constitute it for i.p. administration?

Réponse :
It can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.