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Resveratrol (trans-Resveratrol) HDAC Inhibitor

Name: Resveratrol (trans-Resveratrol)
Brand: Selleck Chemicals
SKU: S1396
Rating: 5 (78 reviews)

제품 번호S1396

Resveratrol은 시클로옥시게나제(예: COX, IC50=1.1 μM), 리폭시게나제(LOX, IC50=2.7 μM), 키나제, 시르투인 및 기타 단백질을 포함한 광범위한 표적을 가지고 있습니다. 항암, 항염증, 혈당 강하 및 기타 유익한 심혈관 효과를 가집니다. Resveratrol은 mitophagy/autophagy 및 자가포식 의존성 apoptosis를 유도합니다.

Resveratrol (trans-Resveratrol) Autophagy 유도제 Chemical Structure

화학 구조

분자량: 228.24

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품질 관리

배치: 순도: 99.99%

99.99

관련 타겟	CXCR Nrf2 Mitophagy LRRK2 ULK FKBP Heme Oxygenase cGAS LC3 Cell wall
기타 Autophagy 억제제	Spautin-1 PIK-III DC661 Lys05 Trihydrochloride Autophinib Spermidine SMER28 EN6 Flubendazole ROC-325

세포 배양, 처리 및 작업 농도

세포주	분석 유형	농도	배양 시간	활성 설명	PMID
human HL60 cell line	Proliferation assay			Antiproliferative activity against human HL60 cell line, IC50=5 μM	16686543
human K562 cell line	Proliferation assay			Antiproliferative activity against human K562 cell line expressing Bcr-Abl, IC50=28 μM	16686543
HUVEC	Function assay	100 μM	1 day	Induction of mitochondrial biogenesis in H2O2-induced HUVEC at 100 umol/L pretreated for 1 day followed by compound washout and H2O2-treatment measured after 10 days by MitoTracker Red staining-based assay	23859249
Hepa1clc7	Cytotoxicity assay		72 hrs	Cytotoxicity against mouse Hepa1clc7 cells after 72 hrs, IC50=11μM.	10075742
MCF7	Function assay		6 hrs	Inhibition of phorbol ester-induced ornithine decarboxylase in human MCF7 cells after 6 hrs, IC50=19μM.	10075742
U937	Function assay			Inhibition of [14C]dehydroascorbic acid uptake in human U937 cells by Michaelis-Menten plot, Ki=35μM.	11277764
U937	Function assay			Inhibition of 2-[1,2-3H(N)]deoxy-D-glucose uptake in human U937 cells by scintillation spectrometry in presence of sodium-free buffer	11277764
HL60	Function assay			Inhibition of 2-[1,2-3H(N)]deoxy-D-glucose uptake in human HL60 cells by scintillation spectrometry in presence of sodium-free buffer	11277764
U937	Function assay			Inhibition of [14C]dehydroascorbic acid uptake in human U937 cells by scintillation spectrometry in presence of sodium-free buffer	11277764
HL60	Function assay			Inhibition of [14C]dehydroascorbic acid uptake in human HL60 cells by scintillation spectrometry in presence of sodium-free buffer	11277764
CHO	Function assay			Blockade of rat Glut1 expressed in CHO cells assessed as inhibition of 2-[1,2-3H(N)]deoxy-D-glucose uptake by scintillation spectrometry	11277764
CHO	Function assay			Blockade of rat Glut1 expressed in CHO cells assessed as inhibition of [14C]dehydroascorbic acid uptake by scintillation spectrometry	11277764
CHO	Function assay			Blockade of human Glut3 expressed in CHO cells assessed as inhibition of 2-[1,2-3H(N)]deoxy-D-glucose uptake by scintillation spectrometry	11277764
CHO	Function assay			Blockade of human Glut3 expressed in CHO cells assessed as inhibition of [14C]dehydroascorbic acid uptake by scintillation spectrometry	11277764
HL60	Function assay			In vitro inhibitory concentration against proliferation of HL60 cells, IC50=5μM.	12877593
RAW264.7	Function assay			Inhibitory activity against nitric oxide production in lipopolysaccharide-activated mouse macrophage RAW264.7 cells, IC50=15.9μM.	14552759
RAW264.7	Function assay			Inhibitory effect on PGE-2 production in LPS-stimulated RAW264.7 cells, IC50=4μM.	15080988
RAW264.7	Function assay			inhibitory concentration against PGE-2 production in LPS-stimulated RAW264.7 cells, IC50=20.8μM.	15501064
T47D	Function assay			Estrogenic activity in human T47D cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol by alamar blue assay, Activity=0.03μM.	15787436
MCF7	Function assay			Estrogenic activity in human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol by alamar blue assay, Activity=1.6μM.	15787436
MDA-MB-231	Antiproliferative assay			Inhibitory concentration required for antiproliferative activity against human MDA-MB-231 cells, IC50=20.5μM.	16250636
U937	Cytotoxicity assay			Cytotoxicity against human U937 cells after 48 hrs by WST1 test, IC50=17μM.	16252924
HL60	Antiproliferative assay			Antiproliferative activity against human HL60 cells, IC50=5μM.	16580204
HEK293T	Function assay			Inhibition of TNF-alpha-induced NF-kappaB activation in HEK293T cells, IC50=20μM.	17125270
BV2	Function assay			Suppression of LPS-stimulated COX2 mRNA expression in mouse BV2 cells at 20 uM	17125270
RAW 264.7	Function assay			Inhibition of LPS and TNF-alpha-activated NO production in murine RAW 264.7 cells, IC50=23.5μM.	17253844
Hepa 1c1c7c1	Function assay	12.5 uM	24 hrs	Induction of quinone reductase in mouse mutant Hepa 1c1c7c1 cells at 12.5 uM after 24 hrs relative to control, Activity=1.16μM.	17316918
Hepa 1c1c7	Function assay	12.5 uM	24 hrs	Induction of quinone reductase activity in mouse Hepa 1c1c7 cells at 12.5 uM after 24 hrs relative to control, Activity=1.24μM.	17316918
MC3T3-E1	Function assay	1 uM		Reversal of increase in ER.DNA complex in mouse MC3T3-E1 cells transfected with dominant-negative mutant Src expression vector at 1 uM by RT-PCR	17513867
MDA-MB-231	Function assay			Reversal of induction of FOXO3a activity in human MDA-MB-231 cells transfected with FOXO3a-specific small interfering RNA	17513867
MDA-MB-231	Function assay			Induction of FOXO3a activity in human MDA-MB-231 cells	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in Src kinase phosphorylation in mouse MC3T3-E1 cells at 1 uM in presence of PP2	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in Src kinase phosphorylation in mouse MC3T3-E1 cells transfected with dominant-negative mutant Src expression vector at 1 uM	17513867
MC3T3-E1	Function assay	1 uM		Increase in Src kinase phosphorylation in mouse MC3T3-E1 cells at 1 uM	17513867
MC3T3-E1	Function assay			Reversal of increase in DNA-binding activity of ERalpha in nuclear extracts of mouse MC3T3-E1 cells by electrophoretic mobility shift assays in presence of short interfering ER alpha-RNA	17513867
MC3T3-E1	Function assay			Reversal of increase in DNA-binding activity of ERalpha in nuclear extracts of mouse MC3T3-E1 cells by electrophoretic mobility shift assays in presence of ICI-182780	17513867
T47D	Antiproliferative assay	1 uM		Antiproliferative activity against human T47D cells at 1 uM by flow cytometry	17513867
primary osteoblast cells	Function assay	1 uM		Reversal of increase in ER.DNA complex in rat primary osteoblast cells transfected with dominant-negative mutant Src expression vector at 1 uM by RT-PCR	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in pBMP2 expression in mouse MC3T3-E1 cells at 1 uM by luciferase method in presence of PP2	17513867
MC3T3-E1	Function assay	1 uM		Induction of ER association with BMP2 promoter in mouse MC3T3-E1 cells at 1 uM by ChiP assay	17513867
MC3T3-E1	Function assay	1 uM		Reversal of induction in nuclear translocation of ERalpha in mouse MC3T3-E1 cells transfected with dominant-negative mutant Src expression vector at 1 uM	17513867
MG63	Function assay	0.1 to 10 uM	24 hr	Increase in proliferation of human MG63 cells at 0.1 to 10 uM after 24 hr by flow cytometry	17513867
MC3T3-E1	Function assay			Increase in DNA-binding activity of ERalpha in nuclear extracts of mouse MC3T3-E1 cells by electrophoretic mobility shift assays	17513867
MCF7	Antiproliferative assay	1 uM		Antiproliferative activity against human MCF7 cells at 1 uM by flow cytometry	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in pBMP2 expression in mouse MC3T3-E1 cells transfected with dominant-negative mutant Src expression vector at 1 uM by luciferase method	17513867
MC3T3-E1	Function assay	0.1 to 10 uM	7 days	Increase in ALP activity in mouse MC3T3-E1 cells at 0.1 to 10 uM after 7 days	17513867
primary osteoblast cells	Function assay	0.1 to 10 uM	7 days	Increase in ALP activity in rat primary osteoblast cells at 0.1 to 10 uM after 7 days	17513867
MC3T3-E1	Function assay	1 uM	72 hrs	Increase in osteocalcin mRNA levels in mouse MC3T3-E1 cells at 1 uM after 72 hrs by RT-PCR	17513867
MG63	Function assay	1 uM	72 hrs	Increase in osteocalcin mRNA levels in human MG63 cells at 1 uM after 72 hrs by RT-PCR	17513867
primary osteoblast cells	Function assay	1 uM	72 hrs	Increase in osteocalcin mRNA levels in rat primary osteoblast cells at 1 uM after 72 hrs by RT-PCR	17513867
MC3T3-E1	Function assay	1 uM	72 hrs	Increase in osteocalcin levels in mouse MC3T3-E1 cells at 1 uM after 72 hrs by ELISA	17513867
MG63	Function assay	1 uM	72 hrs	Increase in osteocalcin levels in human MG63 cells at 1 uM after 72 hrs by ELISA	17513867
primary osteoblast cells	Function assay	1 uM	72 hrs	Increase in osteocalcin levels in rat primary osteoblast cells at 1 uM after 72 hrs by ELISA	17513867
MC3T3-E1	Function assay	1 uM	72 hrs	Increase in osteopontin mRNA levels in mouse MC3T3-E1 cells at 1 uM after 72 hrs by RT-PCR	17513867
MG63	Function assay	1 uM	72 hrs	Increase in osteopontin mRNA levels in human MG63 cells at 1 uM after 72 hrs by RT-PCR	17513867
primary osteoblast cells	Function assay	1 uM	72 hrs	Increase in osteopontin mRNA levels in rat primary osteoblast cells at 1 uM after 72 hrs by RT-PCR	17513867
MC3T3-E1	Function assay	1 uM	72 hrs	Increase in osteopontin levels in mouse MC3T3-E1 cells at 1 uM after 72 hrs by ELISA	17513867
MG63	Function assay	1 uM	72 hrs	Increase in osteopontin levels in human MG63 cells at 1 uM after 72 hrs by ELISA	17513867
primary osteoblast cells	Function assay	1 uM	72 hrs	Increase in osteopontin levels in rat primary osteoblast cells at 1 uM after 72 hrs by ELISA	17513867
MC3T3-E1	Function assay		24 hrs	Inhibition of TNF-alpha-induced apoptosis in mouse MC3T3-E1 cells after 24 hrs by flow cytometry	17513867
primary osteoblast cells	Function assay		24 hrs	Inhibition of etoposide-induced apoptosis in rat primary osteoblast cells after 24 hrs by flow cytometry	17513867
primary osteoblast cells	Function assay		24 hrs	Inhibition of TNF-alpha-induced apoptosis in rat primary osteoblast cells after 24 hrs by flow cytometry	17513867
MCF7	Function assay	1 uM		Reduction in anchorage-independent colony formation of human MCF7 cells at 1 uM by soft agar colony forming assay	17513867
T47D	Function assay	1 uM		Reduction in anchorage-independent colony formation of human T47D cells at 1 uM by soft agar colony forming assay	17513867
primary osteoblast cells	Function assay	1 uM		Increase in BMP2 mRNA levels in rat primary osteoblast cells at 1 uM by RT-PCR method	17513867
primary osteoblast cells	Function assay	1 uM		Reversal of increase in ER.DNA complex in rat primary osteoblast cells at 1 uM by RT-PCR in presence of PP2	17513867
MC3T3-E1	Function assay	1 uM	4 hrs	Increase in BMP2 expression in mouse MC3T3-E1 cells expressing pBMP2 mutant at 1 uM after 4 hrs by luciferase method relative to control	17513867
MC3T3-E1	Function assay	1 uM	4 hrs	Increase in BMP2 expression in mouse MC3T3-E1 cells expressing pBMP2delta1 mutant at 1 uM after 4 hrs by luciferase method relative to control	17513867
MC3T3-E1	Function assay	1 uM		Reversal of induction of BMP2 expression in mouse MC3T3-E1 cells at 1 uM by Western blot in presence of ICI-182780	17513867
MC3T3-E1	Function assay	1 uM		Reversal of induction of BMP2 expression in mouse MC3T3-E1 cells at 1 uM by Western blot in presence of short interfering ERalpha-RNA	17513867
MC3T3-E1	Function assay	1 uM		Reversal of induction in nuclear translocation of ERalpha in mouse MC3T3-E1 cells at 1 uM in presence of PP2	17513867
MC3T3-E1	Function assay	1 uM		Reversal of induction in BMP2 expression in mouse MC3T3-E1 cells transfected with dominant-negative mutant Src expression vector at 1 uM by Western blot	17513867
MC3T3-E1	Function assay	1 uM		Reversal of induction in BMP2 expression in mouse MC3T3-E1 cells at 1 uM by Western blot in presence of PP2	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in ER.DNA complex in mouse MC3T3-E1 cells at 1 uM by RT-PCR in presence of PP2	17513867
primary osteoblast cells	Function assay	1 uM		Reversal of increase in pBMP2 expression in rat primary osteoblast cells transfected with dominant-negative mutant Src expression vector at 1 uM by luciferase method	17513867
primary osteoblast cells	Function assay	1 uM		Reversal of increase in pBMP2 expression in rat primary osteoblast cells at 1 uM by luciferase method in presence of PP2	17513867
MC3T3-E1	Function assay			Reversal of increase in DNA-binding activity of ERalpha in nuclear extracts of mouse MC3T3-E1 cells transfected with dominant-negative mutant Src expression vector by electrophoretic mobility shift assays	17513867
MC3T3-E1	Function assay			Reversal of increase in DNA-binding activity of ERalpha in nuclear extracts of mouse MC3T3-E1 cells by electrophoretic mobility shift assays in presence of PP2	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in BMP2 expression in mouse MC3T3-E1 cells expressing small interfering RNA for FOXA1 protein at 1 uM by Western blot	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in pBMP2 expression in mouse MC3T3-E1 cells expressing small interfering RNA for FOXA1 protein at 1 uM by luciferase method	17513867
MC3T3-E1	Function assay	1 uM		Reversal of increase in ER.DNA complex in mouse MC3T3-E1 cells expressing small interfering RNA for FOXA1 protein at 1 uM by RT-PCR	17513867
MDA-MB-231	Function assay			Reversal of inhibition in anchorage-independent colony formation of human MDA-MB-231 cells transfected with FOXO3a-specific small interfering RNA by soft agar colony forming assay	17513867
MDA-MB-231	Function assay			Inhibition of IGF-induced FOXO3a phosphorylation at Thr32 residue in human MDA-MB-231 cells by Western blot	17513867
MDA-MB-231	Function assay			Inhibition of IGF-induced Akt phosphorylation in human MDA-MB-231 cells by Western blot	17513867
MDA-MB-231	Function assay			Reversal of induction of Akt inactivation in human MDA-MB-231 cells transfected with constitutively activated Akt mutant	17513867
MDA-MB-231	Function assay			Reversal of induction of FOXO3a nuclear accumulation in human MDA-MB-231 cells transfected with constitutively activated Akt mutant	17513867
MDA-MB-231	Function assay			Reversal of induction of FOXO3a activity in human MDA-MB-231 cells transfected with constitutively activated Akt mutant	17513867
MDA-MB-231	Function assay			Enhancement of Akt inactivation in human MDA-MB-231 cells transfected with dominant negative Akt mutant	17513867
MDA-MB-231	Function assay			Enhancement of FOXO3a nuclear accumulation in human MDA-MB-231 cells transfected with dominant negative Akt mutant	17513867
MDA-MB-231	Function assay			Enhancement of FOXO3a activity in human MDA-MB-231 cells transfected with dominant negative Akt mutant	17513867
MDA-MB-231	Function assay	10 mg/kg	2 days	Decrease in Akt activation in human MDA-MB-231 cells expressing FOXO3a-specific small interfering RNA xenografted in nude mice at 10 mg/kg for 2 days	17513867
MDA-MB-231	Function assay	10 mg/kg	2 days	Induction of FOXO3a nuclear accumulation in human MDA-MB-231 cells FOXO3a-specific small interfering RNA xenografted in nude mice at 10 mg/kg for 2 days	17513867
MDA-MB-231	Antiproliferative assay	1 uM		Antiproliferative activity against human MDA-MB-231 cells at 1 uM by flow cytometry	17513867
MDA-MB-231	Function assay	1 uM		Reduction in anchorage-independent colony formation of human MDA-MB-231 cells at 1 uM by soft agar colony forming assay	17513867
MC3T3-E1	Function assay	1 uM	48 hrs	Inhibition of induction of ALP activity in mouse MC3T3-E1 cells at 1 uM after 48 hrs in presence of BMP2-specific neutralizing antibody	17513867
primary osteoblast cells	Function assay	1 uM	48 hrs	Inhibition of induction of ALP activity in rat primary osteoblast cells at 1 uM after 48 hrs in presence of BMP2-specific neutralizing antibody	17513867
MC3T3-E1	Function assay	1 uM	48 hrs	Inhibition of induction of ALP activity in mouse MC3T3-E1 cells at 1 uM after 48 hrs in presence of noggin	17513867
primary osteoblast cells	Function assay	1 uM	48 hrs	Inhibition of induction of ALP activity in rat primary osteoblast cells at 1 uM after 48 hrs in presence of noggin	17513867
primary osteoblast cells	Antiapoptotic activity assay	1 uM	48 hrs	Inhibition of antiapoptotic activity in rat primary osteoblast cells at 1 uM after 48 hrs in presence of BMP2-specific neutralizing antibody	17513867
primary osteoblast cells	Antiapoptotic activity assay	1 uM	48 hrs	Inhibition of antiapoptotic activity in rat primary osteoblast cells at 1 uM after 48 hrs in presence of noggin	17513867
MC3T3-E1	Function assay	0.1 to 10 uM	24 hr	Increase in proliferation of mouse MC3T3-E1 cells assessed as fraction of cells in S phase at 0.1 to 10 uM after 24 hr by flow cytometry	17513867
primary osteoblast cells	Function assay	0.1 to 10 uM	24 hr	Increase in proliferation of rat primary osteoblast cells at 0.1 to 10 uM after 24 hr by flow cytometry	17513867
MG63	Function assay	0.1 to 10 uM	7 days	Increase in ALP activity in human MG63 cells at 0.1 to 10 uM after 7 days	17513867
MC3T3-E1	Function assay		24 hrs	Inhibition of etoposide-induced apoptosis in mouse MC3T3-E1 cells after 24 hrs by flow cytometry	17513867
MC3T3-E1	Antiapoptotic activity assay	1 uM	48 hrs	Inhibition of antiapoptotic activity in mouse MC3T3-E1 cells at 1 uM after 48 hrs in presence of BMP2-specific neutralizing antibody	17513867
MC3T3-E1	Antiapoptotic activity assay	1 uM	48 hrs	Inhibition of antiapoptotic activity in mouse MC3T3-E1 cells at 1 uM after 48 hrs in presence of noggin	17513867
RAW264.7	Function assay			Inhibition of phagocytosis of FITC-conjugated Escherichia coli K12 in mouse RAW264.7 cells assessed as reduction in ratio of number of bacterial cells to phagocytes using trypan blue staining by flow cytometry	17938187
RAW264.7	Function assay			Inhibition of phagocytosis of FITC-conjugated Staphylococcus aureus 209P in mouse RAW264.7 cells assessed as reduction in ratio of number of bacterial cells to phagocytes using trypan blue staining by flow cytometry	17938187
THP1	Function assay			Inhibition of phagocytosis of FITC-conjugated Escherichia coli K12 in human THP1 cells assessed as reduction in ratio of number of bacterial cells to phagocytes using trypan blue staining by flow cytometry	17938187
THP1	Function assay			Inhibition of phagocytosis of FITC-conjugated Staphylococcus aureus 209P in human THP1 cells assessed as reduction in ratio of number of bacterial cells to phagocytes using trypan blue staining by flow cytometry	17938187
THP1	Function assay		1 hr	Inhibition of FSL-1-induced increase in phagocytosis of FITC-conjugated Escherichia coli K12 in human THP1 cells assessed as reduction in number of cells phagocytosing bacteria per cell after 1 hr by CLSM	17938187
THP1	Function assay		1 hr	Inhibition of FSL-1-induced increase in phagocytosis of FITC-conjugated Escherichia coli K12 in human THP1 cells assessed as reduction in number of phagocytosing bacteria to total number of cells after 1 hr by CLSM	17938187
HEK293	Function assay		1 hr	Inhibition of heat killed Escherichia coli K12-induced NF-kappaB activation in HEK293 cells expressing TLR2 pretreated for 1 hr before 6 hrs heat killed bacterial stimulation by luciferase reporter gene assay	17938187
HEK293	Function assay		1 hr	Inhibition of heat killed Staphylococcus aureus 209P-induced NF-kappaB activation in HEK293 cells expressing TLR2 pretreated for 1 hr before 6 hrs heat killed bacterial stimulation by luciferase reporter gene assay	17938187
HEK293	Function assay	100 uM	1 hr	Inhibition of heat killed Escherichia coli K12-induced nuclear translocation of p65 element of NF-kappaB in HEK293 cells expressing TLR2 at 100 uM pretreated for 1 hr before 6 hrs heat killed bacterial stimulation by immunostaining method	17938187
HEK293	Function assay	100 uM	1 hr	Inhibition of heat killed Staphylococcus aureus 209P-induced nuclear translocation of p65 element of NF-kappaB in HEK293 cells expressing TLR2 at 100 uM pretreated for 1 hr before 6 hrs heat killed bacterial stimulation by immunostaining method	17938187
RAW264.7	Function assay	1 to 100 uM	6 hrs	Inhibition of heat killed Escherichia coli K12-induced TNF alpha production in mouse RAW264.7 cells at 1 to 100 uM after 6 hrs by ELISA	17938187
THP1	Function assay	1 to 100 uM	6 hrs	Inhibition of heat killed Escherichia coli K12-induced TNF alpha production in human THP1 cells at 1 to 100 uM after 6 hrs by ELISA	17938187
RAW264.7	Function assay	1 to 100 uM	6 hrs	Inhibition of heat killed Staphylococcus aureus 209P-induced TNF alpha production in mouse RAW264.7 cells at 1 to 100 uM after 6 hrs by ELISA	17938187
THP1	Function assay	1 to 100 uM	6 hrs	Inhibition of heat killed Staphylococcus aureus 209P-induced TNF alpha production in human THP1 cells at 1 to 100 uM after 6 hrs by ELISA	17938187
THP1	Function assay	100 uM	1 hr	Inhibition of FSL1-induced upregulation of MSR1 mRNA expression in in human THP1 cells at 100 uM after 1 hr by RT-PCR	17938187
THP1	Function assay	100 uM	1 hr	Inhibition of FSL1-induced upregulation of CD36 mRNA expression in in human THP1 cells at 100 uM after 1 hr by RT-PCR	17938187
THP1	Function assay	100 uM	1 hr	Inhibition of FSL1-induced upregulation of DC-SIGN mRNA expression in in human THP1 cells at 100 uM after 1 hr by RT-PCR	17938187
THP1	Function assay	100 uM	1 hr	Inhibition of FSL1-induced upregulation of dectin-1 mRNA expression in in human THP1 cells at 100 uM after 1 hr by RT-PCR	17938187
HEK293	Function assay	100 uM		Inhibition of DC-SIGN expression in HEK293 cells expressing DC-SIGN at 100 uM by Western blot analysis	17938187
U2OS	Function assay	100 uM		Activation of SIRT1 in human U2OS cells assessed as decrease in p53 deacetylation level at 100 uM	18046409
293	Function assay		24 hrs	Inhibition of TNFalpha induced NF-kappaB activation in human 293 cells after 24 hrs by luciferase reporter gene assay, IC50=16.1μM.	18487053
cortical microglial	Function assay		48 hrs	Inhibition of LPS-stimulated NO release in rat cortical microglial cells assessed as nitrite accumulation after 48 hrs, IC50=11.5μM.	18588343
HL60	Cytotoxicity assay		48 hrs	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay, IC50=36.3μM.	19251420
PBL	Function assay	12.5 uM		Inhibition of hydrogen peroxide-induced DNA damage in human PBL cells at 12.5 uM by Comet assay	19271734
HL60	Function assay	150 uM	48 hrs	Induction of DNA fragmentation in human HL60 cells at 150 uM after 48 hrs by ethidium bromide based gel electrophoresis	19271734
DLKP	Antiproliferative assay		5 days	Antiproliferative activity against MRP1-expressing human DLKP cells after 5 days by acid phosphatase assay, IC50=10μM.	19481462
DLKP-A	Antiproliferative assay		5 days	Antiproliferative activity against P-gp expressing human DLKP-A cells after 5 days by acid phosphatase assay, IC50=15μM.	19481462
HT144	Antiproliferative assay		5 days	Antiproliferative activity against human HT144 cells after 5 days by acid phosphatase assay, IC50=40μM.	19481462
C2C12	Function assay	50 uM	16 hrs	Increase in mitochondrial function in mouse C2C12 cells assessed as enhancement of mitochondrial density at 50 uM after 16 hrs by green FM dye straining-based fluorescence assay	19663498
3T3L1	Antiadipogenic assay		7 days	Antiadipogenic activity against mouse 3T3L1 cells assessed as inhibition of differentiation after 7 days by oil-red O staining, IC50=31.4μM.	19757853
SW480	Antiproliferative assay		48 hrs	Antiproliferative activity against human SW480 cells assessed as cell viability using propidium iodide staining after 48 hrs, IC50=20μM.	20395019
H460	Antiproliferative assay		72 hrs	Antiproliferative activity against human H460 cells after 72 hrs, IC50=12.9μM.	20409723
H460	Cell cycle assay	20 uM	24 hrs	Cell cycle arrest in human H460 cells assessed as accumulation at G2/M phase at 20 uM after 24 hrs by propidium iodide staining-based flow cytometry	20409723
HEK293	Function assay		48 hrs	Inhibition of TNF-alpha-induced NF-kappaB activity expressed in HEK293 cells after 48 hrs by luciferase reporter gene assay, IC50=0.173μM.	20527891
RAW 264.7	Function assay		20 hrs	Inhibition of iNOS-mediated nitric oxide production in LPS-stimulated mouse RAW 264.7 cells pretreated 30 mins before LPS challenge measured after 20 hrs by Griess reaction method relative to control, IC50=15μM.	20527891
Hepa 1c1c7	Function assay			Inhibition of quinone reductase 1 in mouse Hepa 1c1c7 cells assessed as drug level required to double enzyme activity by MTT assay, CD=21μM.	20527891
RAW264.7	Function assay	34 uM	15 mins	Inhibition of LPS-induced iNOS expression in mouse RAW264.7 cells at 34 uM treated 15 mins before LPS challenge measured after 18 hrs by Western blotting	20527891
Caco-2	Cytotoxicity assay		3 days	Cytotoxicity against human Caco-2 cells after 3 days by [3H]thymidine incorporation assay, IC50=24.35μM.	20627379
HT-29	Cytotoxicity assay		3 days	Cytotoxicity against human HT-29 cells after 3 days by [3H]thymidine incorporation assay, IC50=45.3μM.	20627379
MDA-MB-231	Antitumor assay		6 days	Antitumor activity against human MDA-MB-231 cells after 6 days by SRB assay, IC50=20.5μM.	20728369
CCD-18Co	Antiinflammatory assay	1 uM	18 hrs	Antiinflammatory activity in human CCD-18Co cells assessed inhibition of IL1beta-induced PGE2 production at 1 uM after 18 hrs	20866032
CCD-18Co	Antiinflammatory assay	2.5 to 25 uM	18 hrs	Antiinflammatory activity in human CCD-18Co cells assessed as inhibition of IL1beta-induced PGE2 production at 2.5 to 25 uM after 18 hrs	20866032
Caco2	Function assay		6 hrs	Metabolic stability in human Caco2 cells after 6 hrs by LC-MS-MS analysis	20866032
HT22	Neuroprotective assay		24 hrs	Neuroprotective activity in mouse HT22 cells assessed as protection against glutamate-induced oxytosis after 24 hrs by MTT assay, EC50=4.667μM.	21129978
SW480	Cytotoxicity assay	10 uM	2 days	Cytotoxicity against human SW480 cells at 10 uM for 2 days by vi-cell cell viability analysis	21291235
HCT116	Cytotoxicity assay	10 uM	2 days	Cytotoxicity against human HCT116 cells at 10 uM for 2 days by vi-cell cell viability analysis	21291235
HCT116	Cytotoxicity assay	10 uM	4 days	Cytotoxicity against human HCT116 cells at 10 uM for 4 days by vi-cell cell viability analysis	21291235
LS 174T	Cytotoxicity assay	10 uM	4 days	Cytotoxicity against human LS 174T cells at 10 uM for 4 days by vi-cell cell viability analysis	21291235
LS 174T	Cytotoxicity assay	10 uM	2 days	Cytotoxicity against human LS 174T cells at 10 uM for 2 days by vi-cell cell viability analysis	21291235
LS174T	Function assay	100 uM	36 hrs	Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in Ki67 mRNA level at 100 uM after 36 hrs by RT-PCR analysis	21291235
LS174T	Function assay	100 uM	36 hrs	Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in c-Myc mRNA level at 100 uM after 36 hrs by RT-PCR analysis	21291235
LS174T	Function assay	100 uM	36 hrs	Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in CD44 mRNA level at 100 uM after 36 hrs by RT-PCR analysis	21291235
LS174T	Function assay	100 uM	36 hrs	Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in Lgr5 mRNA level at 100 uM after 36 hrs by RT-PCR analysis	21291235
LS174T	Function assay	100 uM	36 hrs	Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in survivin mRNA level at 100 uM after 36 hrs by RT-PCR analysis	21291235
LS174T	Function assay	100 uM		Inhibition of Wnt/beta-casein in human LS174T cells assessed as increase in p21WAF1/CIP1 protein level at 100 uM by Western blotting	21291235
LS174T	Function assay	100 uM		Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in cyclin B1 protein level at 100 uM by Western blotting	21291235
LS174T	Function assay	100 uM		Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in c-Myc protein level at 100 uM by Western blotting	21291235
LS174T	Function assay	100 uM		Inhibition of Wnt/beta-casein in human LS174T cells assessed as reduction in Cyclin D1 protein level at 100 uM by Western blotting	21291235
HEK293T	Function assay	100 uM	12 hrs	Inhibition of LiCl-induced Wnt/beta-casein in human HEK293T cells transfected with TOP Flash assessed as decrease in luciferase expression at 100 uM after 12 hrs by dual luciferase reporter assay	21291235
SW480	Cytotoxicity assay	10 uM	4 days	Cytotoxicity against human SW480 cells at 10 uM for 4 days by vi-cell cell viability analysis	21291235
LS 174T	Function assay	100 uM		Inhibition of Wnt/beta-casein in human LS 174T cells assessed as reduction in TCF4 protein level at 100 uM by Western blotting	21291235
LS 174T	Function assay	100 uM		Inhibition of Wnt/beta-casein in human LS 174T cells assessed as reduction in pygopus2 protein level at 100 uM by Western blotting	21291235
HEK293T	Function assay	100 uM	12 hrs	Inhibition of Wnt/beta-casein in human HEK293T cells transfected with TOP Flash assessed as decrease in luciferase expression at 100 uM after 12 hrs by dual luciferase reporter assay	21291235
32Dcl3	Function assay	10 uM	1 hr	Radioprotective activity against 0 to 7 Gy gamma radiation-induced death in mouse 32Dcl3 cells at 10 uM treated 1 hr prior to irradiation after 7 days by microscopy	21826253
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs by SRB assay, IC50=0.0000225μM.	21851083
SK-N-SH	Antiproliferative assay		48 hrs	Antiproliferative activity against human SK-N-SH cells assessed as cell viability after 48 hrs by SRB assay, IC50=0.0000403μM.	21851083
A549	Antiproliferative assay		48 hrs	Antiproliferative activity against human A549 cells assessed as cell viability after 48 hrs by SRB assay, IC50=0.0000447μM.	21851083
MCF7	Antiproliferative assay		48 hrs	Antiproliferative activity against human MCF7 cells assessed as cell viability after 48 hrs by SRB assay, IC50=0.0000791μM.	21851083
RAW264.7	Antiinflammatory assay	50 uM	1.5 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNF-alpha production at 50 uM after 1.5 hrs by ELISA	22088309
HEK293	Function assay			Inhibition of TNFalpha-activated NF-kappaB expressed in HEK293 cells by luciferase reporter gene assay, IC50=0.98μM.	22115839
Hepa-1c1c7	Function assay			Induction of QR1 activity in mouse Hepa-1c1c7 cells using MTT as substrate by spectrophotometry, CD=21μM.	22115839
RAW264.7	Antiinflammatory assay		30 mins	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins before LPS challenge measured after 24 hrs by Griess method, IC50=30.71μM.	22115839
B16F10	Antimelanogenic assay	200 uM	72 hrs	Antimelanogenic activity in mouse assessed as cellular melanin content B16F10 cells at 200 uM after 72 hrs spectrophotometric analysis	22189272
HEK293	Function assay			Inhibition of TNF-alpha activated NF-kappaB expressed in HEK293 cells by luciferase reporter gene assay, IC50=0.98μM.	22386564
Hepa-1c1c7	Function assay			Induction of QR1 in mouse Hepa-1c1c7 cells assessed as concentration required to double QR1 induction by MTT assay, CD=21μM.	22386564
RAW264.7	Function assay		18 hrs	Inhibition of iNOS in LPS-stimulated mouse RAW264.7 cells after 18 hrs, IC50=23.2μM.	22386564
RAW264.7	Antiinflammatory assay		30 mins	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production preincubated for 30 mins measured 24 hrs post LPS challenge by Griess method, IC50=30.71μM.	22386564
NIH/3T3	Cytotoxicity assay		96 hrs	Cytotoxicity against mouse NIH/3T3 cells after 96 hrs by SRB assay, IC50=24.22μM.	22749392
HEK293	Function assay			Inhibition of TNFalpha-induced NF-kappaB activity expressed in HEK293 cells by luciferase reporter gene assay, IC50=0.98μM.	23142320
Hepa-1c1c7	Function assay		48 hrs	Induction of quinone reductase-1 activity in mouse Hepa-1c1c7 cells after 48 hrs by MTT assay, CD=21μM.	23142320
RAW 264.7	Function assay		30 mins	Inhibition of LPS-induced iNOS activity in mouse RAW 264.7 cells assessed as inhibition of NO production pretreated with compound for 30 mins before LPS challenge after 24 hrs by Griess reagent method, IC50=23.2μM.	23142320
MCF7	Function assay	>100 uM		Activation of SIRT1 in human MCF7 cells assessed as reduction in doxorubicin-induced acetyl-p53 level at >100 uM after 6 hrs by Western blotting analysis	23316803
HL60	Cytotoxicity assay		48 hrs	Cytotoxicity against human HL60 cells assessed as growth inhibition measured after 48 hrs by XTT assay, IC50=31.8μM.	23547843
HeLa	Function assay			Inhibition of smad3/4 signaling pathway in human HeLa cells assessed as measured as lowest concentration required 50 to 60 % inhibition of TGF-beta induced luciferase treated 30 mins before induction, MED=33μM.	23547843
HeLa	Function assay			Inhibition of Hedgehog signaling pathway in human HeLa cells assessed as measured as lowest concentration required 50 to 60 % inhibition of PMA induced luciferase treated 30 mins before induction, MED=40μM.	23547843
HeLa	Function assay			Inhibition of Hedgehog signaling pathway in human HeLa cells assessed as inhibition of PMA induced luciferase treated 30 mins before induction, IC50=40μM.	23547843
HeLa	Function assay			Inhibition of Notch signaling pathway in human HeLa cells assessed as measured as lowest concentration required 50 to 60 % inhibition of PMA induced luciferase treated 30 mins before induction, MED=40μM.	23547843
HeLa	Function assay			Inhibition of Notch signaling pathway in human HeLa cells assessed as inhibition of PMA induced luciferase treated 30 mins before induction, IC50=40μM.	23547843
SK-MEL	Cytotoxicity assay		48 hrs	Cytotoxicity against human SK-MEL cells assessed as growth inhibition measured after 48 hrs by XTT assay, IC50=47.3μM.	23547843
HL60	Cytotoxicity assay			Cytotoxicity against human HL60 cells, ED50=22μM.	23582778
Huh7.5.1	Cytotoxicity assay		72 hrs	Cytotoxicity against human Huh7.5.1 cells after 72 hrs, IC50=10.6μM.	23673225
MCF7	Cytotoxicity assay		72 hrs	Cytotoxicity against human estrogen receptor positive MCF7 cells after 72 hrs by MTT assay, IC50=28.07μM.	23860590
SKBR3	Cytotoxicity assay		72 hrs	Cytotoxicity against human estrogen receptor negative SKBR3 cells after 72 hrs by MTT assay, IC50=41.42μM.	23860590
ScN2a-cl3	Function assay		5 days	Inhibition of RML prion protein infected in mouse dividing ScN2a-cl3 cells expressing full length mouse PrP assessed as reduction of PrPsc level after 5 days by Western blotting analysis, EC50=0.58μM.	24183589
ScN2a-cl3	Function assay		5 days	Inhibition of RML prion protein infected in mouse dividing ScN2a-cl3 cells expressing full length mouse PrP assessed as reduction of PrPsc level after 5 days by ELISA, EC50=3.06μM.	24183589
SH-SY5Y	Neuroprotective assay	10 uM	24 hrs	Neuroprotective activity in human SH-SY5Y cells assessed as inhibition H2O2-induced reduction of cell viability at 10 uM after 24 hrs by phase-contrast micrograph analysis	24269515
HEK293	Function assay		6 hrs	Inhibition of TNF-alpha-induced NF-kappaB activity in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50=2.5μM.	24992702
Hepa-1c1c7	Function assay		48 hrs	Induction of quinone reductase 1 in mouse Hepa-1c1c7 cells after 48 hrs by MTT assay, CD=21μM.	24992702
HeLa	Function assay	10 to 100 uM	24 hrs	Induction of SV40 promoter-mediated luciferase gene expression in human HeLa cells at 10 to 100 uM after 24 hrs by RT-PCR analysis	25147604
COLO205	Antiproliferative assay			Antiproliferative activity against human COLO205 cells by MTT assay, IC50=23.5μM.	25455486
MGC803	Antiproliferative assay			Antiproliferative activity against human MGC803 cells by MTT assay, IC50=42μM.	25455486
MDA468	Antiproliferative assay			Antiproliferative activity against human MDA468 cells by MTT assay, IC50=45.2μM.	25455486
HeLa	Antiproliferative assay		48 hrs	Antiproliferative activity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay, IC50=47.9μM.	25817772
RAW264.7	Anti-inflammatory assay	10 mM	10 mins	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced IL6 production at 10 mM pre-incubated for 10 mins before LPS stimulation for 24 hrs by ELISA method	26048788
RAW264.7	Anti-inflammatory assay	10 mM	10 mins	Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced TNFalpha production at 10 mM pre-incubated for 10 mins before LPS stimulation for 24 hrs by ELISA method	26048788
HaCaT	Function assay	1 uM	1 hr	Activation of SIRT1 in human HaCaT cells assessed as H4K16 deacetylation at 1 uM after 1 hr by Western blotting method	26689352
HEK293	Function assay			Antagonist activity at rat TRPA1 expressed in HEK293 cells assessed as inhibition of AITC-induced increase of intracellular calcium level, IC50=0.75μM.	26750258
HEK293	Function assay			Agonist activity at rat TRPA1 expressed in HEK293 cells assessed as induction of intracellular calcium level in absence of AITC, IC50=1.4μM.	26750258
HEK293	Function assay			Agonist activity at rat TRPA1 expressed in HEK293 cells assessed as induction of intracellular calcium level in presence of AITC, IC50=2.74μM.	26750258
HEK293	Function assay			Antagonist activity at rat TRPA1 expressed in HEK293 cells assessed as inhibition of allyl isothiocyanate-induced increase of intracellular calcium level, IC50=19.9μM.	26750258
U373-MAGI	Antiviral assay	50 uM	2 hrs	Potentiation of 5-Aza-C-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as 5-Aza-C EC50 at 50 uM preincubated for 2 hrs followed by 5-Aza-C addition for 2 hrs and subsequent viral infection measu, EC50=10μM.	27117260
U373-MAGI	Antiviral assay	50 uM	4 hrs	Antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in viral infectivity at 50 uM incubated for 4 hrs prior to viral infection measured at 72 hrs post infection by flow cytometric analysis	27117260
U373-MAGI	Function assay	200 uM	6 hrs	Reduction in dGTP level in human U373-MAGI cells at 200 uM after 6 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Function assay	200 uM	6 hrs	Reduction in dTTP level in human U373-MAGI cells at 200 uM after 6 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Antiviral assay	25 to 100 uM	2 hrs	Potentiation of 5-Aza-C-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in viral infectivity at 25 to 100 uM preincubated for 2 hrs followed by 5-Aza-C addition for 2 hrs and subsequ	27117260
U373-MAGI	Antiviral assay		2 to 8 hrs	Antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in viral infectivity preincubated for 2 to 8 hrs followed by viral infection measured at 72 hrs post infection by flow cytometric analysis	27117260
U373-MAGI	Function assay	200 uM	6 hrs	Reduction in dCTP level in human U373-MAGI cells at 200 uM after 6 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Function assay	50 uM	2 hrs	Reduction in dCTP level in human U373-MAGI cells at 50 uM preincubated for 2 hrs followed by 5-aza-C addition measured after 4 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Function assay	200 uM	6 hrs	Reduction in dATP level in human U373-MAGI cells at 200 uM after 6 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Function assay	200 uM	2 hrs	Reduction in dRGU-TP level in human U373-MAGI cells at 200 uM preincubated for 2 hrs followed by 5-aza-C addition measured after 4 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Function assay	50 uM	2 hrs	Increase in 5-aza-dCTP/dCTP ratio in human U373-MAGI cells at 50 uM preincubated for 2 hrs followed by 5-aza-dC addition measured after 4 hrs by LC-MS/MS analysis relative to 5-aza-dC	27117260
U373-MAGI	Function assay	200 uM	2 hrs	Reduction in dCTP level in human U373-MAGI cells at 200 uM preincubated for 2 hrs followed by 5-aza-C addition measured after 4 hrs by LC-MS/MS analysis relative to 5-aza-C	27117260
U373-MAGI	Antiviral assay	50 uM	2 to 72 hrs	Potentiation of 5-Aza-C-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in U5-gag level at 50 uM after 2 to 72 hrs by qPCR method	27117260
U373-MAGI	Antiviral assay	50 uM	6 hrs	Antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in gag level at 50 uM measured at 6 hrs post infection by qPCR method	27117260
U373-MAGI	Function assay	200 uM	2 hrs	Increase in 5-aza-dCTP/dCTP ratio in human U373-MAGI cells at 200 uM preincubated for 2 hrs followed by 5-aza-dC addition measured after 4 hrs by LC-MS/MS analysis relative to 5-aza-dC	27117260
U373-MAGI	Function assay	200 uM	2 hrs	Reduction in 5-aza-dCTP level in human U373-MAGI cells at 200 uM preincubated for 2 hrs followed by 5-aza-C addition measured after 4 hrs by LC-MS/MS analysis	27117260
U373-MAGI	Antiviral assay	50 uM	2 to 8 hrs	Potentiation of 5-Aza-C-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in viral infectivity at 50 uM preincubated for 2 to 8 hrs followed by 5-Aza-C addition for 2 hrs and subsequen	27117260
U373-MAGI	Antiviral assay	25 uM	2 hrs	Potentiation of 5-Aza-dC-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in viral infectivity at 25 uM preincubated for 2 hrs followed by 5-Aza-dC addition for 2 hrs and subsequent v	27117260
U373-MAGI	Function assay	50 uM	2 hrs	Increase in 5-aza-dCTP/dCTP ratio in human U373-MAGI cells at 50 uM preincubated for 2 hrs followed by 5-aza-C addition measured after 4 hrs by LC-MS/MS analysis relative to 5-aza-C	27117260
U373-MAGI	Function assay	50 uM	2 hrs	Reduction in dCTP level in human U373-MAGI cells at 50 uM preincubated for 2 hrs followed by 5-aza-dC addition measured after 4 hrs by LC-MS/MS analysis relative to 5-aza-dC	27117260
U373-MAGI	Function assay	200 uM	2 hrs	Reduction in dCTP level in human U373-MAGI cells at 200 uM preincubated for 2 hrs followed by 5-aza-dC addition measured after 4 hrs by LC-MS/MS analysis relative to 5-aza-dC	27117260
U373-MAGI	Antiviral assay	50 uM	2 to 72 hrs	Potentiation of 5-Aza-dC-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in U5-gag level at 50 uM after 2 to 72 hrs by qPCR method	27117260
U373-MAGI	Antiviral assay	50 uM	2 to 72 hrs	Potentiation of 5-Aza-C-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in gag level at 50 uM after 2 to 72 hrs by qPCR method	27117260
U373-MAGI	Antiviral assay	50 uM	2 to 72 hrs	Potentiation of 5-Aza-dC-induced antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in gag level at 50 uM after 2 to 72 hrs by qPCR method	27117260
U373-MAGI	Antiviral assay	50 uM	6 hrs	Antiviral activity against VSV-G pseudotyped HIV-1 NL4-3 infected in human U373-MAGI cells assessed as reduction in U5-gag level at 50 uM measured at 6 hrs post infection by qPCR method	27117260
DH5aplha	Function assay		2 mins	Inhibition of recombinant human CYP1A1 expressed in Escherichia coli DH5aplha cells assessed as O-deethylation of ethoxyresorufin in presence of NADPH measured after 2 mins by spectrofluorometric method, IC50=34μM.	27265259
MDAH 2774	Cytotoxicity assay		72 hrs	Cytotoxicity against human MDAH 2774 cells after 72 hrs by MTT assay, CD50=14.6μM.	27515319
T24	Cytotoxicity assay		72 hrs	Cytotoxicity against human T24 cells after 72 hrs by MTT assay, CD50=22.7μM.	27515319
MDAH 2774	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDAH 2774 cells after 48 hrs by MTT assay, CD50=23μM.	27515319
DU145	Cytotoxicity assay		72 hrs	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay, CD50=27.9μM.	27515319
MHRF	Cytotoxicity assay		72 hrs	Cytotoxicity against human MHRF cells after 72 hrs by MTT assay, CD50=32μM.	27515319
MDAH 2774	Cytotoxicity assay		24 hrs	Cytotoxicity against human MDAH 2774 cells after 24 hrs by MTT assay, CD50=36.9μM.	27515319
HCT116	Cytotoxicity assay	100 uM	48 hrs	Cytotoxicity against human HCT116 cells assessed as cell growth inhibition at 100 uM after 48 hrs by MTT assay	27887843
Bel7402	Cytotoxicity assay	100 uM	48 hrs	Cytotoxicity against human Bel7402 cells assessed as cell growth inhibition at 100 uM after 48 hrs by MTT assay	27887843
HepG2	Function assay		5 hrs	Activation of Nrf2 (unknown origin) expressed in human HepG2 cells after 5 hrs by ARE-driven luciferase reporter gene assay, EC50=5.4μM.	28126440
HEK293	Growth inhibition assay		2 days	Growth inhibition of HEK293 cells after 2 days by MTT assay, IC50=17μM.	28408224
MCF7	Growth inhibition assay		2 days	Growth inhibition of human MCF7 cells after 2 days by MTT assay, IC50=34μM.	28408224
Ramos	Growth inhibition assay		2 days	Growth inhibition of human Ramos cells after 2 days by MTT assay, IC50=37μM.	28408224
ALL5	Cytotoxicity assay		120 hrs	Cytotoxicity against human ALL5 cells after 120 hrs by MTT method, IC50=10.5μM.	28499732
HEK293	Function assay			Inhibition of human TRPA1 expressed in HEK293 cells assessed as decrease in AITC-induced current response by whole cell patch clamp method, IC50=0.75μM.	28576617
LN229	Cytotoxicity assay		48 hrs	Cytotoxicity against human LN229 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=11μM.	28654265
NCI-H460	Cytotoxicity assay		48 hrs	Cytotoxicity against human NCI-H460 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=12μM.	28654265
MML1	Cytotoxicity assay		48 hrs	Cytotoxicity against human MML1 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=12μM.	28654265
BxPC3	Cytotoxicity assay		48 hrs	Cytotoxicity against human BxPC3 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=14μM.	28654265
SK-MEL-2	Cytotoxicity assay		48 hrs	Cytotoxicity against human SK-MEL-2 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=14μM.	28654265
MDA-MB-231	Cytotoxicity assay		48 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=15μM.	28654265
A549	Cytotoxicity assay		48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=16μM.	28654265
U87	Cytotoxicity assay		48 hrs	Cytotoxicity against human U87 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=20μM.	28654265
T47D	Cytotoxicity assay		48 hrs	Cytotoxicity against human T47D cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=25μM.	28654265
HT-29	Cytotoxicity assay		48 hrs	Cytotoxicity against human HT-29 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=27μM.	28654265
COLO201	Cytotoxicity assay		48 hrs	Cytotoxicity against human COLO201 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=37μM.	28654265
PANC1	Cytotoxicity assay		48 hrs	Cytotoxicity against human PANC1 cells assessed as decrease in cell viability after 48 hrs by MTT assay, IC50=39μM.	28654265
A549	Growth inhibition assay			Growth inhibition of human A549 cells, IC50=30μM.	28814374
BV2	Antineuroinflammatory assay		24 hrs	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production after 24 hrs in presence of LPS by Griess reaction based assay, IC50=18.66μM.	28911817
A375	Function assay	25 uM	24 hrs	Inhibition of alpha-MSH-induced TRP-1 expression in human A375 cells at 25 uM after 24 hrs by Western blot method	29150333
A375	Function assay	25 uM	24 hrs	Inhibition of alpha-MSH-induced TRP-2 expression in human A375 cells at 25 uM after 24 hrs by Western blot method	29150333
A375	Function assay	25 uM	4 hrs	Inhibition of alpha-MSH-induced reactive oxygen species generation in human A375 cells at 25 uM after 4 hrs by DCFDA staining-based fluorescence assay	29150333
HEK293T	Cytotoxicity assay		24 hrs	Cytotoxicity against HEK293T cells after 24 hrs by MTT assay, CC50=22.75μM.	29172079
3T3L1	Function assay	1 to 10 uM	6 days	Inhibition of triglyceride accumulation in mouse 3T3L1 cells at 1 to 10 uM after 6 days by ORO staining-based microscopic method	29172079
RAW264.7	Function assay	10 uM	24 hrs	Inhibition of LPS-induced TNF-alpha production in rat RAW264.7 cells at 10 uM after 24 hrs by ELISA	29407944
RAW264.7	Function assay	10 uM	24 hrs	Inhibition of LPS-induced IL-6 production in rat RAW264.7 cells at 10 uM after 24 hrs by ELISA	29407944
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
BT-37	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
BV2	Antineuroinflammatory assay		24 hrs	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced IL-1beta production after 24 hrs by ELISA, IC50=8.7μM.	29499485
BV2	Antineuroinflammatory assay		24 hrs	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced TNFalpha production after 24 hrs by ELISA, IC50=9.5μM.	29499485
BV2	Antineuroinflammatory assay		30 mins	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production pretreated for 30 mins followed by LPS stimulation and measured after 24 hrs by Griess assay, IC50=11.1μM.	29499485
J774	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced IL6 expression after 24 hrs by ELISA, IC50=6.8μM.	29726680
J774	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced nitric oxide production after 24 hrs by Griess assay, IC50=12.15μM.	29726680
J774	Antiinflammatory assay		24 hrs	Antiinflammatory activity in mouse J774 cells assessed as reduction in LPS-induced MCP1 expression after 24 hrs by ELISA, IC50=13.6μM.	29726680
RAW 264.7	Function assay		24 hrs	Inhibition of NFkappaB in mouse RAW 264.7 cells assessed as reduction in LPS induced NO production after 24 hrs in presence of OBHS by Griess assay, IC50=11μM.	30053783
RAW 264.7	Function assay		24 hrs	Inhibition of NFkappaB in mouse RAW 264.7 cells assessed as reduction in LPS induced NO production after 24 hrs by Griess assay, IC50=13.1μM.	30053783
MCF7	Antiproliferative assay		72 hrs	Antiproliferative activity against human MCF7 cells after 72 hrs by MTT assay, IC50=22.9μM.	30053783
MCF7	Antiproliferative assay		72 hrs	Antiproliferative activity against human MCF7 cells after 72 hrs in presence of OBHS by MTT assay, IC50=25.4μM.	30053783
BV2	Antineuroinflammatory assay	10 to 30 uM	24 hrs	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced NO production at 10 to 30 uM after 24 hrs by Griess assay	30096650
RAW264.7	Antiinflammatory assay		1 hr	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS/IFNgamma-induced NO production by measuring NO levels incubated with LPS/IFNgamma for 1 hr followed by compound addition measured after 48 hrs by Griess assay (Rvb = 55 +/- 2., Activity=44.5μM.	30193941
T cells	Antiproliferative assay	50 uM	1 hr	Antiproliferative activity against Con-A-induced T cells isolated from splenocytes of C57/BL6 mouse at 50 uM incubated with Con-A for 1 hr followed by compound addition measured after 72 hrs by MTT assay	30193941
B cells	Antiproliferative assay	50 uM	1 hr	Antiproliferative activity against LPS-induced B cells isolated from splenocytes of C57/BL6 mouse at 50 uM incubated with LPS for 1 hr followed by compound addition measured after 72 hrs by MTT assay	30193941
RAW264.7	Immunomodulatory assay	50 uM	1 hr	Immunomodulatory activity in LPS-stimulated mouse RAW264.7 cells assessed as decrease in CD80 expression at 50 uM incubated with LPS for 1 hr followed by compound addition measured after 24 hrs by flow cytometry	30193941
RAW264.7	Immunomodulatory assay	50 uM	1 hr	Immunomodulatory activity in LPS-stimulated mouse RAW264.7 cells assessed as decrease in CD86 expression at 50 uM incubated with LPS for 1 hr followed by compound addition measured after 24 hrs by flow cytometry	30193941
HBZY-1	Function assay	6 uM		Activation of Nrf2 in rat HBZY-1 cells assessed as increase in NQO-1 level at 6 uM by Western blot analysis	30408749
HBZY-1	Function assay	6 uM		Activation of Nrf2 in rat HBZY-1 cells assessed as increase in HO-1 level at 6 uM by Western blot analysis	30408749
HBZY-1	Function assay	6 uM		Activation of Nrf2 in rat HBZY-1 cells assessed as increase in GCLM level at 6 uM by Western blot analysis	30408749
HBZY-1	Function assay	6 uM		Activation of Nrf2 in rat HBZY-1 cells assessed as increase in GCLC level at 6 uM by Western blot analysis	30408749
HBZY-1	Function assay	6 uM	24 hrs	Activation of Nrf2 in rat HBZY-1 cells assessed as increase in NQO-1 mRNA expression at 6 uM after 24 hrs by RT-PCR analysis	30408749
HBZY-1	Function assay	6 uM	24 hrs	Activation of Nrf2 in rat HBZY-1 cells assessed as increase in HO-1 mRNA expression at 6 uM after 24 hrs by RT-PCR analysis	30408749
HBZY-1	Function assay	6 uM	24 hrs	Activation of Nrf2 in rat HBZY-1 cells assessed as increase in GCLM mRNA expression at 6 uM after 24 hrs by RT-PCR analysis	30408749
HBZY-1	Function assay	6 uM	24 hrs	Activation of Nrf2 in rat HBZY-1 cells assessed as increase in GCLC mRNA expression at 6 uM after 24 hrs by RT-PCR analysis	30408749
BV2	Antineuroinflammatory assay	30 mins	24 hrs	Antineuroinflammatory activity in mouse BV2 cells assessed as inhibition of LPS-induced nitric oxide production pretreated for 30 mins followed by LPS-stimulation for 24 hrs by Griess assay, IC50=11.1μM.	30771601
MCF7	Antioxidant assay	100 uM	24 hrs	Antioxidant activity in human MCF7 cells assessed as decrease in reactive oxygen species generation at 100 uM incubated for 24 hrs by CellROX green/Hoechst 33342 staining based assay	30921758
RAW264.7	Antiinflammatory assay	10 uM	1 hr	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production at 10 uM pretreated for 1 hr followed by LPS stimulation and measured after 24 hrs by Griess reagent based assay	31077997
HBZY-1	Function assay			Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Nrf2 nuclear translocation by measuring increase in ratio of nuclear to cytosolic Nrf2 level by Western blot analysis	31301564
HBZY-1	Function assay			Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in NQO-1 protein expression by Western blot analysis	31301564
HBZY-1	Function assay			Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in HO-1 protein expression by Western blot analysis	31301564
HBZY-1	Function assay			Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in GCLM protein expression by Western blot analysis	31301564
HBZY-1	Function assay			Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in GCLC protein expression by Western blot analysis	31301564
HBZY-1	Function assay	6 uM		Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in NQO-1 mRNA expression at 6 uM by RT-PCR analysis	31301564
HBZY-1	Function assay	6 uM		Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in HO-1 mRNA expression at 6 uM by RT-PCR analysis	31301564
HBZY-1	Function assay	6 uM		Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in GCLM mRNA expression at 6 uM by RT-PCR analysis	31301564
HBZY-1	Function assay	6 uM		Binding affinity to Keap1 in rat HBZY-1 cells assessed as induction of Keap1/Nrf2 signaling by measuring increase in GCLC mRNA expression at 6 uM by RT-PCR analysis	31301564
RAW264.7	Antiinflammatory assay		2 hrs	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS/IFNgamma-induced NO production by measuring NO level preincubated for 2 hrs and followed by LPS/IFNgamma addition and measured after 24 hrs by Griess assay, IC50=44.1μM.	31350127
PMNL	Function assay		15 mins	Inhibition of 5-LOX in human PMNL cells assessed as A23187-stimulated LTB4 production preincubated for 15 mins followed by A23187 addition and measured after 10 mins by HPLC analysis, IC50=4.9μM.	31351395
H9c2	Antioxidant assay	80 nM to 2 uM	18 hrs	Antioxidant activity in rat H9c2 cells assessed as reduction in hypoxia-induced NO release at 80 nM to 2 uM preincubated for 18 hrs followed by incubated in hypoxia for 48 hrs by griess assay	31351395
H9c2	Antioxidant assay	2 uM	18 hrs	Antioxidant activity in rat H9c2 cells assessed as increase in hypoxia-induced reduction of MnSOD expression at 2 uM preincubated for 18 hrs followed by incubated in hypoxia for 48 hrs by Western blot analysis	31351395
TOV112D	Cytotoxicity assay		96 hrs	Cytotoxicity against human TOV112D cells transfected with empty vector assessed as reduction in cell viability incubated for 96 hrs by MTT assay, IC50=10.67μM.	31514018
TOV112D	Cytotoxicity assay		96 hrs	Cytotoxicity against human TOV112D cells expressing NQO2 assessed as reduction in cell viability incubated for 96 hrs by MTT assay, IC50=11μM.	31514018
SKOV3	Cytotoxicity assay		96 hrs	Cytotoxicity against human SKOV3 cells transfected with non-targeted vector assessed as reduction in cell viability incubated for 96 hrs in presence of doxycycline by MTT assay, IC50=29μM.	31514018
SKOV3	Cytotoxicity assay		96 hrs	Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay, IC50=36.3μM.	31514018
SKOV3	Cytotoxicity assay		96 hrs	Cytotoxicity against human SKOV3 cells transfected with shRNA assessed as reduction in cell viability incubated for 96 hrs in presence of doxycycline by MTT assay, IC50=39.67μM.	31514018
HuH7	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human HuH7 cells transfected with ShSGK1 assessed as late apoptosis at 100 uM incubated for 48 hrs by annexin V and 7-AAD staining based assay	31539779
HUH7	Function assay	100 uM	6 hrs	Inhibition of insulin-induced SGK1 in human HUH7 cells at 100 uM using peptide as substrate incubated for 6 hrs by scintillation counter method	31539779
HuH7	Function assay	100 uM	6 hrs	Inhibition of SGK1 in human HuH7 cells assessed as reduction in insulin-induced MDM2 phosphorylation on Ser166 at 100 uM incubated for 6 hrs by Western blot analysis	31539779
HuH7	Antiproliferative assay	100 uM	48 hrs	Antiproliferative activity against human HuH7 cells assessed as reduction in cell viability at 100 uM incubated for 48 hrs by trypan blue dye based assay	31539779
HuH7	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human HuH7 cells assessed as increase in early apoptotic cells at 100 uM incubated for 48 hrs by annexin V and 7-AAD staining based assay	31539779
HuH7	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human HuH7 cells assessed as increase in late apoptotic cells at 100 uM incubated for 48 hrs by annexin V and 7-AAD staining based assay	31539779
HuH7	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human HuH7 cells assessed as increase in necrotic cells at 100 uM incubated for 48 hrs by annexin V and 7-AAD staining based assay	31539779
HuH7	Antiproliferative assay	100 uM	48 hrs	Antiproliferative activity against human HuH7 cells transfected with ShSGK1 assessed as reduction in cell viability at 100 uM incubated for 48 hrs by trypan blue dye based assay	31539779
HuH7	Antiproliferative assay	100 uM	48 hrs	Antiproliferative activity against human HuH7 cells overexpressing EGFP assessed as reduction in cell viability at 100 uM incubated for 48 hrs by trypan blue dye based assay	31539779
HuH7	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human HuH7 cells transfected with ShSGK1 assessed as early apoptosis at 100 uM incubated for 48 hrs by annexin V and 7-AAD staining based assay	31539779
HuH7	Apoptosis assay	100 uM	48 hrs	Induction of apoptosis in human HuH7 cells overexpressing EGFP at 100 uM incubated for 48 hrs by annexin V and 7-AAD staining based assay	31539779
클릭하여 더 많은 세포주 실험 데이터 보기

화학 정보, 보관 및 안정성

분자량	228.24	화학식	C₁₄H₁₂O₃	보관 (수령일로부터)	3년-20°C분말
CAS 번호	501-36-0	SDF 다운로드		원액 보관	1년-80°C용매에 보관 1개월-20°C용매에 보관
동의어	SRT501			Smiles	C1=CC(=CC=C1C=CC2=CC(=CC(=C2)O)O)O

용해도

In vitro
배치:

DMSO : 300 mg/mL (1314.4 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 300 mg/mL (1314.4 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 45 mg/mL (197.16 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 46 mg/mL (201.54 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Water : Insoluble

Ethanol : Insoluble

DMSO : 300 mg/mL (1314.4 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Water : Insoluble

Ethanol : Insoluble

몰농도 계산기

질량	농도	부피	분자량
=	×	×

희석 계산기 분자량 계산기

In vivo 배치:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Selleck 연구소에서 검증되었습니다. 이 제형에 대한 조정이 필요한 경우 맞춤 테스트를 위해 당사 영업팀에 문의하십시오.	15.000mg/ml (65.72mM)	Taking the 1 mL working solution as an example, add 50 μL of 300 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Selleck 연구소에서 검증되었습니다. 이 제형에 대한 조정이 필요한 경우 맞춤 테스트를 위해 당사 영업팀에 문의하십시오.	6.000mg/ml (26.29mM)	Taking the 1 mL working solution as an example, add 50 μL of 120 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Selleck 연구소에서 검증되었습니다. 이 제형에 대한 조정이 필요한 경우 맞춤 테스트를 위해 당사 영업팀에 문의하십시오.	2.300mg/ml (10.08mM)	Taking the 1 mL working solution as an example, add 50 μL of 46 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

생체 내 제형 계산기 (투명한 용액)

1단계: 아래 정보 입력 (권장: 실험 중 손실을 고려하여 추가 동물 포함)

투여량: mg/kg 동물 평균 체중: g 동물당 투여량:μL 동물 수:

2단계: 생체 내 제형 입력 (이것은 계산기일 뿐 제형이 아닙니다. 용해도 섹션에 생체 내 제형이 없는 경우 먼저 당사에 문의하십시오.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

계산 결과:

작업 농도: mg/ml;

DMSO 원액 준비 방법: mg 약물 사전 용해 μL DMSO ( 원액 농도 mg/mL, 농도가 해당 약물 배치의 DMSO 용해도를 초과하는 경우 먼저 당사에 문의하십시오. )

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가μL PEG300, 혼합하고 투명하게 한 다음 추가μL Tween 80, 혼합하고 투명하게 한 다음 추가 μL ddH₂O, 혼합하고 투명하게 합니다.

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가 μL 옥수수 기름, 혼합하고 투명하게 합니다.

참고: 1. 다음 용매를 추가하기 전에 액체가 투명한지 확인하십시오.
2. 용매를 순서대로 추가해야 합니다. 다음 용매를 추가하기 전에 이전 추가에서 얻은 용액이 투명한 용액인지 확인해야 합니다. 와동, 초음파 또는 뜨거운 물 중탕과 같은 물리적 방법을 사용하여 용해를 도울 수 있습니다.

작용 메커니즘

Targets/IC₅₀/Ki	SIRT1 (Cell-free assay) SIRT2 (Cell-free assay) Quinone reductase 2 (Cell-free assay) 88 nM IKK β (Cell-free assay) 1 μM COX1 (Cell-free assay) 1.1 μM COX2 (Cell-free assay) 1.3 μM DNA polymerase α (Cell-free assay) 3.3 μM LOX (Cell-free assay) 3.7 μM STAT3 (Cell-free assay) 20 μM Src (Cell-free assay) 20 μM ERK1 (Cell-free assay) 37 μM JNK1 (Cell-free assay) 50 μM
시험관 내(In vitro)	Resveratrol은 시클로옥시게나아제와 리폭시게나아제, PKCs 및 p56^lck, ERK1, JNK1, p38, IKK β, Src, STAT3, 리보뉴클레오타이드 리덕타아제, DNA 폴리메라아제 α 및 δ, PKD, PKC α, 퀴논 리덕타아제 2, 그리고 아로마타아제의 활성을 IC50이 0.035-60 μM 범위에서 억제합니다. 이 화합물은 또한 아데닐릴 시클라아제와 AMPK의 활성제로, 각각 EC50이 0.8 μM 및 50 μM입니다. 이 화합물이 억제제 또는 활성제로서의 역할은 세포 염증 관련 행동 감소, 암세포의 성장 억제 및 apoptosis 유도, 엔도텔린-1에 의해 자극된 세포 반응의 역전, 포르볼 에스테르 유도 COX-2 발현 억제, 세포 내 DNA 합성 억제, 메나디온 유도 세포 사멸에 대한 저항성, 그리고 세포 미토콘드리아 기능 및 포도당/지질 대사 개선에 대한 효과를 가능하게 합니다. 또한 sirtuins의 활성제이기도 합니다. 이 화학물질은 아세틸화된 기질과 NAD⁺ 모두에 대한 SIRT1의 미카엘리스 상수를 낮추고, SIRT1 의존성 p53 탈아세틸화를 자극하여 세포 생존율을 높입니다. 효모에서는 Sir2를 자극하여 칼로리 제한을 모방하고, DNA 안정성을 높이며 수명을 연장합니다. 이 화합물은 항산화 활성을 통해 허혈/재관류 손상으로부터 분리된 쥐 심장을 보호하는 데 효과적이며, 발달된 압력과 대동맥류 흐름의 회복 개선, 말론디알데하이드 농도 감소 및 경색 크기 감소를 보였습니다.
생체 내(In vivo)	Resveratrol은 고칼로리 식이를 하는 쥐의 건강과 생존율을 향상시킵니다. 이 화합물(22.4 mg/kg/일)은 고칼로리 식이를 하는 중년 쥐의 생리를 표준 식이를 하는 쥐의 생리로 전환시키고 생존율을 유의하게 증가시킵니다. 이는 인슐린 민감도 증가, 인슐린 유사 성장 인자-1(IGF-I) 수치 감소, AMP 활성 단백질 키나제(AMPK) 및 퍼옥시좀 증식자 활성화 수용체-γ 보조활성인자 1α(PGC-1α) 활성 증가, 미토콘드리아 수 증가, 운동 기능 향상 등 장수와 관련된 변화를 유발합니다. 이 물질은 153개의 유의하게 변경된 경로 중 144개에서 고칼로리 식이에 대한 효과를 상쇄했습니다. 다양한 설치류 암 모델에서 종양의 개시 및 성장을 억제하는 것으로 나타났습니다. 쥐의 결장 암 발생 모델에서 하루 200 μg/kg만큼 낮은 용량의 이 화학 물질도 이미 효능을 보입니다. 40 mg/kg의 더 높은 용량에서는 피하 신경모세포종을 가진 쥐의 생존율을 0%에서 70%로 증가시킵니다. 이 화합물은 매일 투여했을 때 단 48 μg/kg 용량으로 쥐의 각막 미세주머니 분석에서 혈관신생을 억제합니다. 심장 질환에 유익한 효과를 보입니다. 이 물질은 고콜레스테롤혈증 식단으로 유발된 혈소판 응집 증가를 차단합니다. 이는 내피 및 유도성 산화질소 합성효소의 발현을 증가시킵니다. 뇌졸중 취약성, 자발적 고혈압 쥐에서 Resveratrol은 혈청의 글리코실화 알부민 및 소변의 8-하이드록시구아노신과 같은 산화 스트레스 마커를 유의하게 감소시킵니다. 15일 동안(1 mg/kg) 이 화학 물질을 음용수에 제공하는 것만으로도 분리된 심장의 기능 및 관상동맥 흐름 회복을 개선하기에 충분합니다. 생체 내에서 항염증 활성을 나타냅니다. 이 화합물은 급성 및 만성 화학적으로 유도된 부종, 리포다당류 유도 기도 염증 및 골관절염을 유의하게 감소시키고, 동종이식 거부 반응을 예방하는 데 도움을 줍니다. 정맥 투여된 Resveratrol은 허혈/재관류, 하이포크산틴/잔틴 산화효소(HX/XO) 또는 혈소판 활성화 인자에 의해 생성된 산화제에 의해 유도된 염증을 감소시키지만, 쥐에서 류코트리엔 B4에 의한 염증은 감소시키지 않습니다. 뇌졸중 및 뇌 손상에 유익한 효과를 보입니다. 쥐의 중대뇌동맥 폐색 후 100 ng/kg 및 1 μg/kg의 극히 낮은 용량으로 정맥 투여된 물질은 허혈성 용적 및 뇌 수분 함량을 유의하게 감소시켰습니다.
참조	[1] https://pubmed.ncbi.nlm.nih.gov/18421779/ [2] https://pubmed.ncbi.nlm.nih.gov/12939617/ [3] https://pubmed.ncbi.nlm.nih.gov/16732220/ [4] https://pubmed.ncbi.nlm.nih.gov/17086191/ [5] https://pubmed.ncbi.nlm.nih.gov/12887737/ [6] https://pubmed.ncbi.nlm.nih.gov/11895924/ [7] https://pubmed.ncbi.nlm.nih.gov/19738051/ [8] https://pubmed.ncbi.nlm.nih.gov/16611627/ [9] https://pubmed.ncbi.nlm.nih.gov/20450491/

적용 분야

방법	바이오마커	이미지	PMID
Western blot	Nanog / Oct-4 / Sox-2 p-EGFR / EGFR / p-HER2 / HER2 / p-ERK / ERK / PTEN		21304978
Growth inhibition assay	Cell viability		23272133

임상시험 정보

(데이터 출처 https://clinicaltrials.gov, 업데이트 날짜 2024-05-22)

NCT 번호	모집	조건	스폰서/협력자	시작일	단계
NCT05684848	Recruiting	Wheezing\|Recurrent Respiratory Tract Infections	Noos S.r.l.	November 1 2022	Not Applicable
NCT03743636	Completed	Peripheral Artery Disease	Northwestern University\|American Heart Association	October 1 2018	Phase 3

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