Home PI3K/Akt/mTOR mTOR 억제제 Torin 1

연구용

Torin 1 mTOR 억제제

제품 번호S2827

Torin 1은 무세포 분석에서 IC50이 2 nM/10 nM인 강력한 mTORC1/2 억제제이며, PI3K보다 mTOR에 대해 1000배 선택성을 보입니다.
Torin 1 mTOR 억제제 Chemical Structure

화학 구조

분자량: 607.62

바로가기

품질 관리

배치: 순도: 99.07%
99.07

세포 배양, 처리 및 작업 농도

세포주 분석 유형 농도 배양 시간 제형 활성 설명 PMID
ETK-1 Growth Inhibition Assay IC50=0.000306 μM SANGER
OCUB-M Growth Inhibition Assay IC50=0.00194 μM SANGER
DOHH-2 Growth Inhibition Assay IC50=0.00225 μM SANGER
NOS-1 Growth Inhibition Assay IC50=0.00242 μM SANGER
L-363 Growth Inhibition Assay IC50=0.00284 μM SANGER
RL95-2 Growth Inhibition Assay IC50=0.0032 μM SANGER
BL-70 Growth Inhibition Assay IC50=0.00337 μM SANGER
TGBC1TKB Growth Inhibition Assay IC50=0.00379 μM SANGER
697 Growth Inhibition Assay IC50=0.00634 μM SANGER
RPMI-8226 Growth Inhibition Assay IC50=0.00651 μM SANGER
BL-41 Growth Inhibition Assay IC50=0.00696 μM SANGER
MRK-nu-1 Growth Inhibition Assay IC50=0.00707 μM SANGER
EW-1 Growth Inhibition Assay IC50=0.00749 μM SANGER
EVSA-T Growth Inhibition Assay IC50=0.00892 μM SANGER
HUTU-80 Growth Inhibition Assay IC50=0.00929 μM SANGER
VA-ES-BJ Growth Inhibition Assay IC50=0.00984 μM SANGER
GCIY Growth Inhibition Assay IC50=0.01007 μM SANGER
ES6 Growth Inhibition Assay IC50=0.01083 μM SANGER
NALM-6 Growth Inhibition Assay IC50=0.01102 μM SANGER
LB996-RCC Growth Inhibition Assay IC50=0.01137 μM SANGER
EW-16 Growth Inhibition Assay IC50=0.01192 μM SANGER
KALS-1 Growth Inhibition Assay IC50=0.01198 μM SANGER
OPM-2 Growth Inhibition Assay IC50=0.0129 μM SANGER
KU812 Growth Inhibition Assay IC50=0.01296 μM SANGER
A4-Fuk Growth Inhibition Assay IC50=0.01319 μM SANGER
KGN Growth Inhibition Assay IC50=0.01359 μM SANGER
KS-1 Growth Inhibition Assay IC50=0.01392 μM SANGER
no-11 Growth Inhibition Assay IC50=0.01395 μM SANGER
PSN1 Growth Inhibition Assay IC50=0.01409 μM SANGER
SK-LMS-1 Growth Inhibition Assay IC50=0.01413 μM SANGER
LS-411N Growth Inhibition Assay IC50=0.01439 μM SANGER
HT-144 Growth Inhibition Assay IC50=0.01451 μM SANGER
ACN Growth Inhibition Assay IC50=0.015 μM SANGER
RKO Growth Inhibition Assay IC50=0.01533 μM SANGER
CMK Growth Inhibition Assay IC50=0.01543 μM SANGER
GB-1 Growth Inhibition Assay IC50=0.01553 μM SANGER
KE-37 Growth Inhibition Assay IC50=0.01573 μM SANGER
no-10 Growth Inhibition Assay IC50=0.01584 μM SANGER
MOLT-16 Growth Inhibition Assay IC50=0.01622 μM SANGER
HEL Growth Inhibition Assay IC50=0.0164 μM SANGER
NCI-H1355 Growth Inhibition Assay IC50=0.01646 μM SANGER
IST-MEL1 Growth Inhibition Assay IC50=0.01774 μM SANGER
MHH-PREB-1 Growth Inhibition Assay IC50=0.01818 μM SANGER
ES8 Growth Inhibition Assay IC50=0.01844 μM SANGER
LC-2-ad Growth Inhibition Assay IC50=0.02137 μM SANGER
LAMA-84 Growth Inhibition Assay IC50=0.02167 μM SANGER
BV-173 Growth Inhibition Assay IC50=0.02203 μM SANGER
SW872 Growth Inhibition Assay IC50=0.02247 μM SANGER
SK-UT-1 Growth Inhibition Assay IC50=0.02261 μM SANGER
OS-RC-2 Growth Inhibition Assay IC50=0.02323 μM SANGER
NCI-H747 Growth Inhibition Assay IC50=0.0233 μM SANGER
MZ2-MEL Growth Inhibition Assay IC50=0.02337 μM SANGER
TE-11 Growth Inhibition Assay IC50=0.02372 μM SANGER
KURAMOCHI Growth Inhibition Assay IC50=0.02414 μM SANGER
HD-MY-Z Growth Inhibition Assay IC50=0.02426 μM SANGER
GI-1 Growth Inhibition Assay IC50=0.02469 μM SANGER
LB1047-RCC Growth Inhibition Assay IC50=0.02488 μM SANGER
TE-12 Growth Inhibition Assay IC50=0.0255 μM SANGER
MZ7-mel Growth Inhibition Assay IC50=0.02609 μM SANGER
MMAC-SF Growth Inhibition Assay IC50=0.02633 μM SANGER
A101D Growth Inhibition Assay IC50=0.0265 μM SANGER
GR-ST Growth Inhibition Assay IC50=0.02655 μM SANGER
MC116 Growth Inhibition Assay IC50=0.0268 μM SANGER
Ramos-2G6-4C10 Growth Inhibition Assay IC50=0.0275 μM SANGER
ATN-1 Growth Inhibition Assay IC50=0.02789 μM SANGER
MFH-ino Growth Inhibition Assay IC50=0.02812 μM SANGER
DEL Growth Inhibition Assay IC50=0.02815 μM SANGER
OCI-AML2 Growth Inhibition Assay IC50=0.02871 μM SANGER
LXF-289 Growth Inhibition Assay IC50=0.02872 μM SANGER
IA-LM Growth Inhibition Assay IC50=0.02917 μM SANGER
EoL-1-cell Growth Inhibition Assay IC50=0.02996 μM SANGER
OVCAR-4 Growth Inhibition Assay IC50=0.03094 μM SANGER
ES1 Growth Inhibition Assay IC50=0.03097 μM SANGER
CESS Growth Inhibition Assay IC50=0.03099 μM SANGER
GAK Growth Inhibition Assay IC50=0.03186 μM SANGER
NTERA-S-cl-D1 Growth Inhibition Assay IC50=0.03226 μM SANGER
TE-10 Growth Inhibition Assay IC50=0.03311 μM SANGER
CCRF-CEM Growth Inhibition Assay IC50=0.03371 μM SANGER
NMC-G1 Growth Inhibition Assay IC50=0.03448 μM SANGER
HOP-62 Growth Inhibition Assay IC50=0.03556 μM SANGER
SH-4 Growth Inhibition Assay IC50=0.03582 μM SANGER
NCI-H1648 Growth Inhibition Assay IC50=0.03622 μM SANGER
EB-3 Growth Inhibition Assay IC50=0.03643 μM SANGER
BB65-RCC Growth Inhibition Assay IC50=0.03691 μM SANGER
BE-13 Growth Inhibition Assay IC50=0.03759 μM SANGER
CP67-MEL Growth Inhibition Assay IC50=0.03759 μM SANGER
SW954 Growth Inhibition Assay IC50=0.0379 μM SANGER
TE-5 Growth Inhibition Assay IC50=0.03854 μM SANGER
HCE-4 Growth Inhibition Assay IC50=0.03855 μM SANGER
LC-1F Growth Inhibition Assay IC50=0.03989 μM SANGER
TK10 Growth Inhibition Assay IC50=0.04019 μM SANGER
SJSA-1 Growth Inhibition Assay IC50=0.04188 μM SANGER
Daudi Growth Inhibition Assay IC50=0.04225 μM SANGER
SW982 Growth Inhibition Assay IC50=0.04246 μM SANGER
SF126 Growth Inhibition Assay IC50=0.04361 μM SANGER
TE-15 Growth Inhibition Assay IC50=0.04435 μM SANGER
COLO-684 Growth Inhibition Assay IC50=0.04561 μM SANGER
8-MG-BA Growth Inhibition Assay IC50=0.04629 μM SANGER
NB13 Growth Inhibition Assay IC50=0.04776 μM SANGER
HCC1599 Growth Inhibition Assay IC50=0.04879 μM SANGER
SK-PN-DW Growth Inhibition Assay IC50=0.04949 μM SANGER
ALL-PO Growth Inhibition Assay IC50=0.04956 μM SANGER
NB6 Growth Inhibition Assay IC50=0.05271 μM SANGER
IST-SL2 Growth Inhibition Assay IC50=0.05362 μM SANGER
BB30-HNC Growth Inhibition Assay IC50=0.0573 μM SANGER
SW962 Growth Inhibition Assay IC50=0.05763 μM SANGER
TGBC24TKB Growth Inhibition Assay IC50=0.05799 μM SANGER
KINGS-1 Growth Inhibition Assay IC50=0.05803 μM SANGER
HC-1 Growth Inhibition Assay IC50=0.05827 μM SANGER
RS4-11 Growth Inhibition Assay IC50=0.05938 μM SANGER
LS-123 Growth Inhibition Assay IC50=0.05942 μM SANGER
A3-KAW Growth Inhibition Assay IC50=0.06225 μM SANGER
SNB75 Growth Inhibition Assay IC50=0.06341 μM SANGER
SR Growth Inhibition Assay IC50=0.06374 μM SANGER
LOUCY Growth Inhibition Assay IC50=0.06394 μM SANGER
K5 Growth Inhibition Assay IC50=0.06403 μM SANGER
Becker Growth Inhibition Assay IC50=0.0642 μM SANGER
CTV-1 Growth Inhibition Assay IC50=0.06548 μM SANGER
KLE Growth Inhibition Assay IC50=0.06584 μM SANGER
MZ1-PC Growth Inhibition Assay IC50=0.06598 μM SANGER
MOLT-4 Growth Inhibition Assay IC50=0.06605 μM SANGER
MLMA Growth Inhibition Assay IC50=0.0678 μM SANGER
A388 Growth Inhibition Assay IC50=0.06893 μM SANGER
MV-4-11 Growth Inhibition Assay IC50=0.06995 μM SANGER
DSH1 Growth Inhibition Assay IC50=0.07173 μM SANGER
MONO-MAC-6 Growth Inhibition Assay IC50=0.07212 μM SANGER
ARH-77 Growth Inhibition Assay IC50=0.07278 μM SANGER
LB771-HNC Growth Inhibition Assay IC50=0.07549 μM SANGER
PF-382 Growth Inhibition Assay IC50=0.07569 μM SANGER
KM12 Growth Inhibition Assay IC50=0.0764 μM SANGER
HAL-01 Growth Inhibition Assay IC50=0.07658 μM SANGER
RPMI-8402 Growth Inhibition Assay IC50=0.07789 μM SANGER
OMC-1 Growth Inhibition Assay IC50=0.07804 μM SANGER
HT Growth Inhibition Assay IC50=0.08 μM SANGER
QIMR-WIL Growth Inhibition Assay IC50=0.08055 μM SANGER
KNS-42 Growth Inhibition Assay IC50=0.08203 μM SANGER
BC-3 Growth Inhibition Assay IC50=0.08204 μM SANGER
ES4 Growth Inhibition Assay IC50=0.08296 μM SANGER
NCI-H23 Growth Inhibition Assay IC50=0.0844 μM SANGER
LB2518-MEL Growth Inhibition Assay IC50=0.08696 μM SANGER
LC4-1 Growth Inhibition Assay IC50=0.08738 μM SANGER
SIG-M5 Growth Inhibition Assay IC50=0.08904 μM SANGER
LP-1 Growth Inhibition Assay IC50=0.08938 μM SANGER
TE-8 Growth Inhibition Assay IC50=0.09016 μM SANGER
K-562 Growth Inhibition Assay IC50=0.0912 μM SANGER
J-RT3-T3-5 Growth Inhibition Assay IC50=0.09153 μM SANGER
DU-4475 Growth Inhibition Assay IC50=0.09165 μM SANGER
RXF393 Growth Inhibition Assay IC50=0.09462 μM SANGER
NB5 Growth Inhibition Assay IC50=0.09625 μM SANGER
A704 Growth Inhibition Assay IC50=0.09741 μM SANGER
SF268 Growth Inhibition Assay IC50=0.09825 μM SANGER
UACC-257 Growth Inhibition Assay IC50=0.09833 μM SANGER
L-428 Growth Inhibition Assay IC50=0.1032 μM SANGER
ST486 Growth Inhibition Assay IC50=0.10606 μM SANGER
SK-MM-2 Growth Inhibition Assay IC50=0.10709 μM SANGER
COR-L279 Growth Inhibition Assay IC50=0.10832 μM SANGER
JiyoyeP-2003 Growth Inhibition Assay IC50=0.10843 μM SANGER
GT3TKB Growth Inhibition Assay IC50=0.10918 μM SANGER
HCE-T Growth Inhibition Assay IC50=0.1094 μM SANGER
SBC-1 Growth Inhibition Assay IC50=0.11119 μM SANGER
KNS-81-FD Growth Inhibition Assay IC50=0.11273 μM SANGER
NCI-H2126 Growth Inhibition Assay IC50=0.11394 μM SANGER
EM-2 Growth Inhibition Assay IC50=0.11529 μM SANGER
JAR Growth Inhibition Assay IC50=0.11598 μM SANGER
NB17 Growth Inhibition Assay IC50=0.11652 μM SANGER
LB647-SCLC Growth Inhibition Assay IC50=0.11705 μM SANGER
CAL-148 Growth Inhibition Assay IC50=0.118 μM SANGER
K052 Growth Inhibition Assay IC50=0.11851 μM SANGER
KARPAS-299 Growth Inhibition Assay IC50=0.12233 μM SANGER
KARPAS-422 Growth Inhibition Assay IC50=0.12432 μM SANGER
LOXIMVI Growth Inhibition Assay IC50=0.12515 μM SANGER
SCC-3 Growth Inhibition Assay IC50=0.12851 μM SANGER
LU-139 Growth Inhibition Assay IC50=0.12975 μM SANGER
COLO-320-HSR Growth Inhibition Assay IC50=0.13086 μM SANGER
ONS-76 Growth Inhibition Assay IC50=0.1313 μM SANGER
U-266 Growth Inhibition Assay IC50=0.13245 μM SANGER
HH Growth Inhibition Assay IC50=0.13535 μM SANGER
BB49-HNC Growth Inhibition Assay IC50=0.13587 μM SANGER
NCI-SNU-5 Growth Inhibition Assay IC50=0.13596 μM SANGER
C2BBe1 Growth Inhibition Assay IC50=0.13746 μM SANGER
ML-2 Growth Inhibition Assay IC50=0.13953 μM SANGER
SCH Growth Inhibition Assay IC50=0.13974 μM SANGER
NCI-H82 Growth Inhibition Assay IC50=0.14104 μM SANGER
MDA-MB-134-VI Growth Inhibition Assay IC50=0.14252 μM SANGER
SK-N-DZ Growth Inhibition Assay IC50=0.14719 μM SANGER
HL-60 Growth Inhibition Assay IC50=0.1492 μM SANGER
LB373-MEL-D Growth Inhibition Assay IC50=0.15539 μM SANGER
LB831-BLC Growth Inhibition Assay IC50=0.16076 μM SANGER
SK-NEP-1 Growth Inhibition Assay IC50=0.16101 μM SANGER
IMR-5 Growth Inhibition Assay IC50=0.16483 μM SANGER
Calu-6 Growth Inhibition Assay IC50=0.16537 μM SANGER
NCI-H1882 Growth Inhibition Assay IC50=0.16728 μM SANGER
NCI-H446 Growth Inhibition Assay IC50=0.16891 μM SANGER
GI-ME-N Growth Inhibition Assay IC50=0.1802 μM SANGER
MPP-89 Growth Inhibition Assay IC50=0.18121 μM SANGER
CGTH-W-1 Growth Inhibition Assay IC50=0.18436 μM SANGER
MEG-01 Growth Inhibition Assay IC50=0.18628 μM SANGER
NCI-H748 Growth Inhibition Assay IC50=0.18911 μM SANGER
COLO-829 Growth Inhibition Assay IC50=0.20673 μM SANGER
GOTO Growth Inhibition Assay IC50=0.2073 μM SANGER
EW-3 Growth Inhibition Assay IC50=0.21718 μM SANGER
KY821 Growth Inhibition Assay IC50=0.22045 μM SANGER
CTB-1 Growth Inhibition Assay IC50=0.22228 μM SANGER
TALL-1 Growth Inhibition Assay IC50=0.22921 μM SANGER
NCI-H209 Growth Inhibition Assay IC50=0.22962 μM SANGER
IST-MES1 Growth Inhibition Assay IC50=0.2351 μM SANGER
DMS-114 Growth Inhibition Assay IC50=0.23993 μM SANGER
SNU-C2B Growth Inhibition Assay IC50=0.24316 μM SANGER
DG-75 Growth Inhibition Assay IC50=0.26195 μM SANGER
D-336MG Growth Inhibition Assay IC50=0.26557 μM SANGER
KMOE-2 Growth Inhibition Assay IC50=0.27538 μM SANGER
NB1 Growth Inhibition Assay IC50=0.27926 μM SANGER
RH-1 Growth Inhibition Assay IC50=0.27989 μM SANGER
TE-1 Growth Inhibition Assay IC50=0.28479 μM SANGER
RPMI-6666 Growth Inhibition Assay IC50=0.2884 μM SANGER
TE-9 Growth Inhibition Assay IC50=0.28985 μM SANGER
BT-474 Growth Inhibition Assay IC50=0.29334 μM SANGER
COLO-668 Growth Inhibition Assay IC50=0.29429 μM SANGER
NCI-H1581 Growth Inhibition Assay IC50=0.29689 μM SANGER
MN-60 Growth Inhibition Assay IC50=0.29905 μM SANGER
L-540 Growth Inhibition Assay IC50=0.30081 μM SANGER
MOLT-13 Growth Inhibition Assay IC50=0.30183 μM SANGER
NCI-SNU-16 Growth Inhibition Assay IC50=0.30205 μM SANGER
NCI-H64 Growth Inhibition Assay IC50=0.30331 μM SANGER
CP66-MEL Growth Inhibition Assay IC50=0.31415 μM SANGER
NB69 Growth Inhibition Assay IC50=0.32188 μM SANGER
NCI-H2196 Growth Inhibition Assay IC50=0.33036 μM SANGER
MHH-CALL-2 Growth Inhibition Assay IC50=0.33468 μM SANGER
HCC2157 Growth Inhibition Assay IC50=0.33954 μM SANGER
LS-1034 Growth Inhibition Assay IC50=0.36388 μM SANGER
BOKU Growth Inhibition Assay IC50=0.37199 μM SANGER
GDM-1 Growth Inhibition Assay IC50=0.37234 μM SANGER
TGW Growth Inhibition Assay IC50=0.37453 μM SANGER
SNU-C1 Growth Inhibition Assay IC50=0.38215 μM SANGER
LS-513 Growth Inhibition Assay IC50=0.39071 μM SANGER
NOMO-1 Growth Inhibition Assay IC50=0.39971 μM SANGER
MS-1 Growth Inhibition Assay IC50=0.40177 μM SANGER
EKVX Growth Inhibition Assay IC50=0.44461 μM SANGER
SK-MEL-2 Growth Inhibition Assay IC50=0.464 μM SANGER
HCC1187 Growth Inhibition Assay IC50=0.46768 μM SANGER
HCC2218 Growth Inhibition Assay IC50=0.47227 μM SANGER
NCI-H187 Growth Inhibition Assay IC50=0.4784 μM SANGER
DB Growth Inhibition Assay IC50=0.50055 μM SANGER
NCI-H69 Growth Inhibition Assay IC50=0.50247 μM SANGER
NCI-H1694 Growth Inhibition Assay IC50=0.50697 μM SANGER
CA46 Growth Inhibition Assay IC50=0.50917 μM SANGER
EHEB Growth Inhibition Assay IC50=0.51193 μM SANGER
NCCIT Growth Inhibition Assay IC50=0.54321 μM SANGER
NCI-H720 Growth Inhibition Assay IC50=0.54962 μM SANGER
LU-134-A Growth Inhibition Assay IC50=0.55296 μM SANGER
MC-CAR Growth Inhibition Assay IC50=0.55431 μM SANGER
NEC8 Growth Inhibition Assay IC50=0.56172 μM SANGER
NCI-H524 Growth Inhibition Assay IC50=0.58748 μM SANGER
NCI-H1963 Growth Inhibition Assay IC50=0.6258 μM SANGER
SU-DHL-1 Growth Inhibition Assay IC50=0.64526 μM SANGER
NCI-H1522 Growth Inhibition Assay IC50=0.64883 μM SANGER
NCI-H1155 Growth Inhibition Assay IC50=0.6535 μM SANGER
D-263MG Growth Inhibition Assay IC50=0.66101 μM SANGER
TE-6 Growth Inhibition Assay IC50=0.68104 μM SANGER
NCI-H1417 Growth Inhibition Assay IC50=0.69711 μM SANGER
NCI-H510A Growth Inhibition Assay IC50=0.77236 μM SANGER
KARPAS-45 Growth Inhibition Assay IC50=0.78235 μM SANGER
IST-SL1 Growth Inhibition Assay IC50=0.85825 μM SANGER
COLO-824 Growth Inhibition Assay IC50=0.87835 μM SANGER
NCI-H526 Growth Inhibition Assay IC50=0.90958 μM SANGER
NCI-H719 Growth Inhibition Assay IC50=0.96657 μM SANGER
SF539 Growth Inhibition Assay IC50=0.98396 μM SANGER
NKM-1 Growth Inhibition Assay IC50=0.98663 μM SANGER
A253 Growth Inhibition Assay IC50=0.98885 μM SANGER
TE-441-T Growth Inhibition Assay IC50=1.0435 μM SANGER
COR-L88 Growth Inhibition Assay IC50=1.11354 μM SANGER
CPC-N Growth Inhibition Assay IC50=1.15888 μM SANGER
NCI-H2107 Growth Inhibition Assay IC50=1.17261 μM SANGER
ECC4 Growth Inhibition Assay IC50=1.18693 μM SANGER
NCI-H2141 Growth Inhibition Assay IC50=1.19299 μM SANGER
NCI-H1770 Growth Inhibition Assay IC50=1.32928 μM SANGER
NCI-SNU-1 Growth Inhibition Assay IC50=1.3304 μM SANGER
LU-165 Growth Inhibition Assay IC50=1.45467 μM SANGER
Raji Growth Inhibition Assay IC50=1.47259 μM SANGER
EW-24 Growth Inhibition Assay IC50=1.54619 μM SANGER
KP-N-RT-BM-1 Growth Inhibition Assay IC50=1.56551 μM SANGER
SK-MEL-1 Growth Inhibition Assay IC50=1.64877 μM SANGER
SCLC-21H Growth Inhibition Assay IC50=1.66651 μM SANGER
SIMA Growth Inhibition Assay IC50=1.68233 μM SANGER
NCI-H1304 Growth Inhibition Assay IC50=1.70765 μM SANGER
REH Growth Inhibition Assay IC50=1.71078 μM SANGER
KP-N-YS Growth Inhibition Assay IC50=1.93551 μM SANGER
DMS-153 Growth Inhibition Assay IC50=1.9759 μM SANGER
NB10 Growth Inhibition Assay IC50=2.20878 μM SANGER
NCI-H345 Growth Inhibition Assay IC50=2.61905 μM SANGER
SK-N-FI Growth Inhibition Assay IC50=2.75381 μM SANGER
NCI-H1092 Growth Inhibition Assay IC50=2.95394 μM SANGER
KM-H2 Growth Inhibition Assay IC50=3.65396 μM SANGER
KG-1 Growth Inhibition Assay IC50=3.71688 μM SANGER
D-283MED Growth Inhibition Assay IC50=3.7587 μM SANGER
LU-65 Growth Inhibition Assay IC50=4.11485 μM SANGER
MSTO-211H Growth Inhibition Assay IC50=4.18898 μM SANGER
NCI-H2081 Growth Inhibition Assay IC50=4.29174 μM SANGER
D-247MG Growth Inhibition Assay IC50=4.43191 μM SANGER
P31-FUJ Growth Inhibition Assay IC50=4.45019 μM SANGER
DMS-79 Growth Inhibition Assay IC50=4.93183 μM SANGER
KASUMI-1 Growth Inhibition Assay IC50=4.97509 μM SANGER
JVM-3 Growth Inhibition Assay IC50=5.25014 μM SANGER
LAN-6 Growth Inhibition Assay IC50=5.51736 μM SANGER
DJM-1 Growth Inhibition Assay IC50=5.56029 μM SANGER
JVM-2 Growth Inhibition Assay IC50=5.67092 μM SANGER
U-87-MG Growth Inhibition Assay IC50=5.89964 μM SANGER
SHP-77 Growth Inhibition Assay IC50=5.94928 μM SANGER
RPMI-8866 Growth Inhibition Assay IC50=6.27013 μM SANGER
ES7 Growth Inhibition Assay IC50=6.39924 μM SANGER
WSU-NHL Growth Inhibition Assay IC50=6.51609 μM SANGER
CAS-1 Growth Inhibition Assay IC50=7.09749 μM SANGER
MHH-NB-11 Growth Inhibition Assay IC50=8.23152 μM SANGER
ES5 Growth Inhibition Assay IC50=9.52286 μM SANGER
HDLM-2 Growth Inhibition Assay IC50=9.95902 μM SANGER
NCI-H2171 Growth Inhibition Assay IC50=10.5927 μM SANGER
LNCaP-Clone-FGC Growth Inhibition Assay IC50=11.3718 μM SANGER
NCI-H1395 Growth Inhibition Assay IC50=12.2699 μM SANGER
NCI-H1436 Growth Inhibition Assay IC50=13.3195 μM SANGER
RCC10RGB Growth Inhibition Assay IC50=13.3221 μM SANGER
SW684 Growth Inhibition Assay IC50=13.9797 μM SANGER
NCI-H1650 Growth Inhibition Assay IC50=15.0447 μM SANGER
EW-13 Growth Inhibition Assay IC50=15.2021 μM SANGER
EC-GI-10 Growth Inhibition Assay IC50=15.8644 μM SANGER
CHP-126 Growth Inhibition Assay IC50=17.1918 μM SANGER
NCI-H226 Growth Inhibition Assay IC50=18.0403 μM SANGER
NCI-H1838 Growth Inhibition Assay IC50=20.1848 μM SANGER
EW-11 Growth Inhibition Assay IC50=24.2718 μM SANGER
EB2 Growth Inhibition Assay IC50=31.5318 μM SANGER
D-502MG Growth Inhibition Assay IC50=43.9229 μM SANGER
U-698-M Growth Inhibition Assay IC50=48.8671 μM SANGER
ES3 Growth Inhibition Assay IC50=49.3893 μM SANGER
PC3 Function assay Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM. 21322566
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells 29435139
클릭하여 더 많은 세포주 실험 데이터 보기

화학 정보, 보관 및 안정성

분자량 607.62 화학식

C35H28F3N5O2

 보관 (수령일로부터)
CAS 번호 1222998-36-8 SDF 다운로드 원액 보관

동의어 N/A Smiles CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F

용해도

In vitro
배치:

DMSO : 3 mg/mL (4.93 mM)
(수분으로 오염된 DMSO는 용해도를 감소시킬 수 있습니다. 신선하고 무수 DMSO를 사용하십시오.)

Water : Insoluble

Ethanol : Insoluble

몰농도 계산기

질량 농도 부피 분자량
희석 계산기 분자량 계산기

In vivo
배치:

생체 내 제형 계산기 (투명한 용액)

1단계: 아래 정보 입력 (권장: 실험 중 손실을 고려하여 추가 동물 포함)

mg/kg g μL

2단계: 생체 내 제형 입력 (이것은 계산기일 뿐 제형이 아닙니다. 용해도 섹션에 생체 내 제형이 없는 경우 먼저 당사에 문의하십시오.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

계산 결과:

작업 농도: mg/ml;

DMSO 원액 준비 방법: mg 약물 사전 용해 μL DMSO ( 원액 농도 mg/mL, 농도가 해당 약물 배치의 DMSO 용해도를 초과하는 경우 먼저 당사에 문의하십시오. )

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가μL PEG300, 혼합하고 투명하게 한 다음 추가μL Tween 80, 혼합하고 투명하게 한 다음 추가 μL ddH2O, 혼합하고 투명하게 합니다.

생체 내 제형 준비 방법: 취하다 μL DMSO 원액, 다음 추가 μL 옥수수 기름, 혼합하고 투명하게 합니다.

참고: 1. 다음 용매를 추가하기 전에 액체가 투명한지 확인하십시오.
2. 용매를 순서대로 추가해야 합니다. 다음 용매를 추가하기 전에 이전 추가에서 얻은 용액이 투명한 용액인지 확인해야 합니다. 와동, 초음파 또는 뜨거운 물 중탕과 같은 물리적 방법을 사용하여 용해를 도울 수 있습니다.

작용 메커니즘

Targets/IC50/Ki
mTORC1
(Cell-free assay)
2 nM
mTOR
(Cell-free assay)
4.32 nM
DNA-PK
(Cell-free assay)
6.34 nM
mTORC2
(Cell-free assay)
10 nM
p110γ
(Cell-free assay)
171 nM
C2α
(Cell-free assay)
176 nM
시험관 내(In vitro)
Torin1은 세포 내에서 2 nM 및 10 nM 농도에서 각각 mTORC1 및 mTORC2 기질의 인산화를 억제합니다. 또한, Torin1은 PI3K(EC50 = 1800 nM)보다 mTOR에 대해 1000배 더 높은 선택성을 보이며, 다른 450개 단백질 키나아제에 비해 100배 더 높은 결합 선택성을 나타냅니다. Torin1은 라파마이신 저항성 기전을 통해 세포 주기 정지를 유발하며, 이는 mTORC2와도 독립적입니다. Torin1은 라파마이신보다 mTORC1 의존적 표현형을 더 완전하게 방해합니다. mTORC1의 라파마이신 저항성 기능은 캡 의존적 번역에 필수적입니다. 최근 연구에서는 Torin1이 인간 내분비 세포주 BON에서 MEK/ERK/c-Jun 경로의 활성화를 통해 뉴로텐신 분비와 유전자 발현을 증가시킨다고 보고되었습니다.
키나아제 분석
mTORC1 및 mTORC2 In Vitro 키나아제 분석
가용성 mTORC1을 생산하기 위해 N-말단에 FLAG-태그된 Raptor를 안정적으로 발현하는 HEK-293T 세포주는 수포성 구내염 바이러스 G-슈도타이핑 MSCV 레트로바이러스를 사용하여 생성됩니다. mTORC2의 경우, N-말단에 FLAG-태그된 Protor-1을 안정적으로 발현하는 유사한 HeLa 세포가 생성됩니다. 두 복합체는 50 mm HEPES, pH 7.4, 10 mm 피로인산나트륨, 10 mm β-글리세로포스페이트나트륨, 100 mm NaCl, 2 mm EDTA, 0.3% CHAPS에서 세포를 용해하여 정제됩니다. 세포는 4 °C에서 30분 동안 용해되고, 불용성 분획은 13,000 rpm으로 10분 동안 미세원심분리하여 제거됩니다. 상층액은 FLAG-M2 단일클론 항체-아가로스와 1시간 동안 배양된 후 용해 버퍼로 세 번, 최종 농도 0.5 M NaCl을 포함하는 용해 버퍼로 한 번 세척됩니다. 정제된 mTORC1은 50 mm HEPES, pH 7.4, 100 mm NaCl에 100 μg/mL 3×FLAG 펩타이드를 사용하여 용출됩니다. 용출액은 분주하여 -80 °C에 보관할 수 있습니다. 기질 S6K1 및 Akt1이 정제됩니다. 키나아제 분석은 30 °C에서 20분 동안 20 μL의 최종 부피로 수행되며, 키나아제 버퍼(25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) 및 150 ng의 불활성 S6K1 또는 Akt1을 기질로 포함합니다. 반응은 80 μL의 샘플 버퍼를 첨가하여 중단하고 5분 동안 끓입니다. 샘플은 이어서 SDS-PAGE 및 면역블로팅으로 분석됩니다.
생체 내(In vivo)
Torin1은 U87MG 이종이식 모델에서 20 mg/kg 용량으로 효과적이며, 종양 및 말초 조직에서 mTOR의 하류 이펙터에 대한 우수한 약력학적 억제를 보여줍니다.
참조
  • [4] https://pubmed.ncbi.nlm.nih.gov/20508131/

적용 분야

방법 바이오마커 이미지 PMID
Western blot Vinculin / p-S6 p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b
S2827-WB4
28757207
Immunofluorescence lipin-1
S2827-IF1
28347999
Growth inhibition assay Cell viability
S2827-viability1
28757207

기술 지원

취급 설명서

Tel: +1-832-582-8158 Ext:3

다른 문의사항이 있으시면 메시지를 남겨주세요.

이름을 입력해주세요.
이메일을 입력해주세요. 유효한 이메일 주소를 입력해주세요.
저희에게 무엇이든 작성해주세요.

자주 묻는 질문

질문 1:
How to re-constitute it for i.p. administration?

답변:
It can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.